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. 2018 Aug 29;23(9):2178. doi: 10.3390/molecules23092178

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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ASI (25 mg/kg) increased the total number of newly generated neurons (BrdU⁺/NeuN⁺). (A–I) Confocal images of BrdU (red) and NeuN (green) immunostaining: (A–C) Control group; (D–F) ASI group; and (G–I) enlarged images of the cells from ASI group. (A–F) Scale bar = 100 μm. (G–I) Scale bar = 2 μm. (J) Quantification of BrdU⁺/NeuN⁺ double immunopositive cells in two groups (n = 4/group). (K) Western blotting analysis of NeuN in hippocampus. (L) Gray intensity analysis of NeuN in hippocampus (n = 8/group). * p < 0.05. The data are presented as mean ± SD.