Table 6.
Reusability studies of biosilicified enzymes for esterification reactions with different alcohols under CH.
| Reuse | Conversion to Ethyl Valerate (mol%) | Specific Activity (U/gbiocatalyst) | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| MeOH | EtOH | IPrOH | BuOH | Free Enzyme | MeOH | EtOH | iPrOH | BuOH | Free Enzyme | |
| R0 | - | 95 ± 8 | 92 ± 7 | 89 ± 8 | 82 ± 6 | 418 ± 14 | 506 ± 16 | 508 ± 14 | 427 ± 12 | 352 ± 12 |
| R1 | - | 88 ± 7 | 42 ± 7 | 80 ± 9 | 75 ± 8 | 207 ± 12 | 505 ± 12 | 400 ± 19 | 307 ± 14 | 307 ± 16 |
| R2 | - | 94 ± 6 | - | 70 ± 7 | 68 ± 5 | - | 415 ± 18 | 367 ± 15 | 270 ± 12 | 301 ± 15 |
| R3 | - | 92 ± 8 | - | 69 ± 9 | 60 ± 8 | - | 400 ± 14 | - | 254 ± 17 | 272 ± 9 |
| R4 | - | 93 ± 6 | - | 60 ± 7 | 61 ± 6 | - | 403 ± 11 | - | 207 ± 16 | 260 ± 13 |
Reaction conditions: 12% m/v biosilicified enzyme (equal quantity for free enzyme using buffer solution), 1000 rpm, 40 °C, 2 h CH in batch reactor.