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. 2018 Aug 22;23(9):2109. doi: 10.3390/molecules23092109

Figure 2.

Figure 2

The DegP protease can mediate transpeptidation with proper nucleophiles. (A) A model substrate (18–58; 20 µM) was cleaved by DegP (2 µM) in the presence of ethylenediamine or hydroxylamine (0.25–2 M), and the reaction products were analyzed by MALDI-TOF-MS (N-term, N-terminal fragment; C-term, C-terminal fragment; PA, probe-addition product). Representative spectra are shown (n = 3). (B,C) The MALDI analysis of the products of the same cleavage reactions as in (A), but in the presence of various dipeptides (200 mM; (B); n = 1) or various concentrations of the KF dipeptide (5–80 mM; (C)). Representative spectra are shown in (C) (n = 3).