Table 2.
Proteins detected on two-dimensional electrophoresis gels of ungerminated wheat grains and wheat grain samples germinated for various times (24, 48, 72, and 96 h).
| Spot | Identified Proteins | Accession | Score 2 | Mw | PI | Relative Expression of Proteins | ||||
|---|---|---|---|---|---|---|---|---|---|---|
| Control | 24 h | 48 h | 72 h | 96 h | ||||||
| 3104 | Cytosolic malate dehydrogenase | AAP7009.1 | 98 | 22 | 6.0 | 3313 | 3459 | 2553 | 2355 | 1601 |
| 4011 | Dimeric alpha-amylase inhibitor | ABI54569.1 | 88 | 14 | 6.6 | 215 | 176 | 87 | 77 | ND |
| 4101 | Hypothetical protein CL3131Contig 1 | AFG48690.1 | 69 | 24 | 6.3 | 339 | 204 | 188 | 191 | ND |
| 4010 | Alpha-amylase inhibitor | CAI84642.1 | 96 | 14 | 6.6 | 279 | 202 | 183 | 187 | ND |
| 4008 | Alpha-amylase/trypsin inhibitor | AQT26482.1 | 94 | 12 | 6.6 | 322 | 213 | 265 | 214 | ND |
| 5401 | Phospholipase SGR2isoform | XP_022027330.1 | 86 | 38 | 6.8 | 505 | 499 | 361 | 430 | 262 |
| 1603 | NI 1 | - | 56 | 5.3 | 139 | 153 | 122 | 85 | 85 | |
| 2813 | NI 1 | - | 88 | 5.6 | 753 | 1009 | 1926 | 1854 | 1854 | |
| 3708 | NI 1 | - | 63 | 5.7 | 191 | 192 | 99 | 142 | 537 | |
| 3405 | Granule bound starch synthase | AF75531.1 | 399 | 41 | 6.0 | 2189 | 2363 | 2362 | 2362 | 12,768 |
| 8009 | Dimeric alpha-amylase inhibitor | AAV39517.1 | 153 | 11 | 8.2 | 380 | 1823 | 1367 | 1780 | 2599 |
| 9004 | Glutathione S-transferase | XP_02255327.1 | 77 | 11 | 9.9 | 2581 | 5723 | 6173 | 6058 | 9776 |
| 4201 | unnamed protein product | CDJ26374.1 | 91 | 27 | 6.4 | 414 | 672 | 794 | 586 | 954 |
| 8013 | NI 1 | - | 11 | 8.9 | ND | 1939 | 2940 | 1884 | 3846 | |
| 1801 | Beta-glucosidase | XP_010066854.1 | 63 | 102 | 5.0 | 585 | 895 | 782 | 626 | 442 |
1 NI means not identified by Peptide Mass Fingerprinting. 2 Score indicates the Mowse score from mascot search results, and all scores are significant (p < 0.05). Values of relative expressions of proteins were means of two replications.