Skip to main content
. 2018 Nov 7;100(3):651–668.e8. doi: 10.1016/j.neuron.2018.08.037

Figure 3.

Figure 3

MBON-ɑ2sc Is Functionally Connected to PD2a1 and PD2b1

(A–C) GRASP signal in the dorsal LH (green circles, dashed lines indicate midlines) for the experimental genotype (A) and two controls (B and C). Genotypes and controls are represented in the schematics above each figure. Images are representative of n = 3.

(D) GCaMP6f was expressed in PD2a1 and PD2b1 neurons with the R37G11-GAL4 driver (scale bar, 10 μm). Fluorescence was recorded in vivo from the axonal compartment of PD2a1 and PD2b1 neurons while the temperature was shifted from 20°C to 31°C (dashed line on F, except for the blue trace).

(E) The calcium increase of PD2a1 and PD2b1 neurons because of thermal activation of V2 MBONs (red trace) was stronger than that because of temperature shift only in the genotypic controls (green and purple traces).

(F) Quantification of calcium increase from the traces (n = 10 flies per condition, except 71D08-LexA/+ [n = 8], F(3,37) = 9.09, p = 0.0001).

∗∗p < 0.01. Data are presented as mean ± SEM. See also Figure S6.