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. 2018 Oct 29;7:e38829. doi: 10.7554/eLife.38829

Figure 4. Aberrant recovery of a single neuron-driven behavior.

(A) Diagram of the optogenetically triggered tail-bending behavior. (B) Images from an example movie showing the dorsal tail-bending behavior. (C–H) Quantification of the tail-bending behavior of intact animals (C), axotomized animals 5 min after axotomy (D), 12 hr after axotomy (E), 24 hr after axotomy (F), 48 hr after axotomy (G) and 72 hr after axotomy (H). Both averaged data (upper, Mean ± SEM) and heat maps (lower) of individual responses are shown. Shaded areas represent the 5 s light stimulation. The color bar indicates the tail angle. Positive numbers indicate dorsal bending, and negative numbers indicate ventral bending. (I) Averaged tail angle during the 5 s stimulation at different timepoints after axotomy of animals in (C–H). Mean and SEM. **p<0.01; ***p<0.001; ****p<0.0001; ns, not significant. Unpaired t test. (J and K) Recovery of dorsal bending is abolished 12 hr after the second cut of the regenerating axon (K) but stays the same in the absence of the second cut (J). ****p<0.0001; ns, not significant. Paired t test.

Figure 4.

Figure 4—figure supplement 1. Optogenetically-triggered tail-bending behavior requires Chrimson and ATR and is DA9 specific.

Figure 4—figure supplement 1.

(A) ric-7(n2657) animals expressing mCherry instead of Chrimson supplied with ATR show no dorsal tail bending. Mean ± SEM. The shaded area represents the 5 s stimulation. (B) ric-7(n2657) animals expressing Chrimson in the absence of ATR show no dorsal tail bending. Mean ± SEM. The shaded area represents the 5 s stimulation. (C) WT animals expressing Chrimson supplied with ATR but with their DA9 cell body ablated show no dorsal tail bending. Mean ± SEM. The shaded area represents the 5 s stimulation. (D) Averaged tail angle during stimulation of positive controls and animals in (A), (B) and (C). The positive controls are the same as in Figure 4C. Mean and SEM. ****p<0.0001. Unpaired t test.
Figure 4—figure supplement 2. Optogenetically-triggered tail-bending behavior depends on synaptic transmission.

Figure 4—figure supplement 2.

(A) Tail-bending behavior of intact unc-13(e51) animals (2-day-old adults). (B) Tail-bending behavior of unc-13(e51) animals 48 hr after axotomy. (C) Averaged tail angle during stimulation of both intact and axotomized unc-13(e51) animals in (A) and (B). ns, not significant. Unpaired t test.