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. 2018 Nov 12;20(12):1236–1245. doi: 10.1016/j.neo.2018.10.006

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© 2018 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Survival analysis of F10 and Chk1 inhibition by PF-477736.

Colony survival assay performed in (A) HCT-116 and (C) HT-29 cells shows decreased survival of Chk1i treated colonies co-treated with F10 compared to DMSO-treated cells co-treated with F10. Western blot analysis shows decreased pChk1-317 and increased γH2AX in the combination in both (B) HCT-116 and (D) HT-29 cell lines. Additionally, decreased expression of Rad51 and ubiquitination of FANCD2 were observed in both the cell lines treated with PF-477736.