Note that while anti-NGF increased the size of the calcified callus at days 14, 21 and 42 post-fracture (A) by day 63 post-fracture the difference between the two groups had disappeared. Also, note anti-NGF did not appear to impact cortical union (B). For (A-B), the level of significance between Fracture + Vehicle and Fracture + Anti-NGF animals are indicated by *, **, *** = P< 0.05, 0.01 and 0.001, respectively.