Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information | ||
|---|---|---|---|---|---|---|
| Cell line (Human) | 786-O | purchased from ATCC | ATCC CRL-1932 | |||
| Cell line (Human) | A498 | purchased from ATCC | ATCC CRL-7908 | |||
| Cell line (Human) | Ren-01 | Daniel Lindner at Cleveland Clinic |
||||
| Cell line (Human) | Ren-02 | Daniel Lindner at Cleveland Clinic |
||||
| Cell line (Human) | RCC4 | William Kaelin Jr. at Dana-Farber Cancer Institute |
||||
| Cell line (Human) | Caki-1 | William Kaelin Jr. at Dana-Farber Cancer Institute |
||||
| Cell line (Human) | HEK293T | William Kaelin Jr. at Dana-Farber Cancer Institute |
||||
| Cell line (Human) | UMRC2 | Qing Zhang at UNC Chapel Hill |
||||
| Cell line (Human) | UMRC6 | Qing Zhang at UNC Chapel Hill |
||||
| Antibody | BAP1 (mouse monoclonal) | Santa Cruz Biotech, sc-28383 |
(1:200 for western blot, 1:50 for IHC) |
|||
| Antibody | STAT2 (rabbit polyclonal) | Bethyl A303-512A | (1:1,000 for western blot, 1:25 for IHC) |
|||
| Antibody | IRF9 (rabbit polyclonal) | Sigma, HPA001862 | (1:2,000 for western blot, 1:50 for IHC) |
|||
| Recombinant DNA reagent | Flag-PBRM1 (human) | This paper | Available upon request. | |||
| Recombinant DNA reagent | Flag-KDM5C (human) | This paper | Available upon request. | |||
| Other | Ren-02 cells with BAP1 knocked out | This paper | BAP1 gene was knocked out with the CrRISPR-Cas9 technique. Detailed description was listed in the Materials and methods. |
|||