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. 2018 Jul 11;37(46):6041–6053. doi: 10.1038/s41388-018-0403-0

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Effects of MUC20 knockdown on PDAC cells in vitro. a The mRNA levels of MUC20 analysed by real-time RT-PCR in PDAC cell lines, as indicated. b The protein levels of MUC20 analysed by Western blotting in PDAC cell lines. c Western blots showing MUC20 knockdown with two independent siRNAs (si-MUC20-1 and si-MUC20-2) in HPAC and HPAF-II cells. d MUC20 knockdown inhibited viability in HPAC and HPAF-II cells analysed by MTT assays. *P < 0.05 e MUC20 knockdown did not significantly affect 10% FBS-triggered migration and invasion in HPAC and HPAF-II cells analysed by Transwell migration and Matrigel invasion assay, respectively. Data are presented as mean (n = 3) ± SD