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. 2018 Nov 19;8:17007. doi: 10.1038/s41598-018-35398-0

Differential toxicities of fine particulate matters from various sources

Minhan Park 1, Hung Soo Joo 1,2, Kwangyul Lee 1, Myoseon Jang 3, Sang Don Kim 1, Injeong Kim 1, Lucille Joanna S Borlaza 1, Heungbin Lim 4, Hanjae Shin 5, Kyu Hyuck Chung 6, Yoon-Hyeong Choi 7, Sun Gu Park 7, Min-Suk Bae 8, Jiyi Lee 9, Hangyul Song 1, Kihong Park 1,✉
PMCID: PMC6242998  PMID: 30451941

Abstract

Fine particulate matters less than 2.5 µm (PM2.5) in the ambient atmosphere are strongly associated with adverse health effects. However, it is unlikely that all fine particles are equally toxic in view of their different sizes and chemical components. Toxicity of fine particles produced from various combustion sources (diesel engine, gasoline engine, biomass burning (rice straw and pine stem burning), and coal combustion) and non-combustion sources (road dust including sea spray aerosols, ammonium sulfate, ammonium nitrate, and secondary organic aerosols (SOA)), which are known major sources of PM2.5, was determined. Multiple biological and chemical endpoints were integrated for various source-specific aerosols to derive toxicity scores for particles originating from different sources. The highest toxicity score was obtained for diesel engine exhaust particles, followed by gasoline engine exhaust particles, biomass burning particles, coal combustion particles, and road dust, suggesting that traffic plays the most critical role in enhancing the toxic effects of fine particles. The toxicity ranking of fine particles produced from various sources can be used to better understand the adverse health effects caused by different fine particle types in the ambient atmosphere, and to provide practical management of fine particles beyond what can be achieved only using PM mass which is the current regulation standard.

Introduction

Fine particles in the ambient atmosphere are of significant research interest owing to their impacts on climate change and detrimental effects on human health. These particles are directly emitted from various sources and produced by gas-to-particle conversion of secondary products from atmospheric oxidation of SO2, NOx, and hydrocarbons. Epidemiological and toxicological studies worldwide have suggested a strong link between exposure to fine particles and adverse health effects (e.g., respiratory disease, lung cancer, cardiovascular disease, and premature mortality)1–3. The World Health Organization (WHO) established guidelines for particulate matter based on mass concentration of particulate matter less than 2.5 µm (PM2.5)4. However, health risks of PM2.5 are not fully taken into account with the PM2.5 mass, and all fine particles may not be equally toxic5. Due to multiple sources and formation pathways, ambient PM2.5 have diverse sizes, shapes, surface charges, surface chemistry and chemical compositions, leading to differential health effects among particle types. Even different PM2.5 with the same mass concentrations may exert variable effects on human health. For example, an earlier study by Lelieveld et al.3 in their assessment of the contribution of outdoor air pollution sources to premature mortality reported that health impact was dependent on assumptions on toxicity of particles6,7. A recent review on epidemiological and toxicological literatures related to long-term effects of PM components or source factors suggests that there has been insufficient information to make clear conclusions about differential health effects among components or sources8. It was also reported that much more enhanced understanding of exposure and health effects should be needed before it can be concluded that the control of specific sources or components of PM2.5 should be more effective to protect human health than the PM2.5 mass as a whole9.

The differential toxicities among particle types according to chemical composition remain to be established due to the existence of numerous chemical constituents in ambient aerosols. Conduction of complete toxicity tests for all possible chemical constituents of ambient aerosols and generation of practical chemical groups for regulatory purposes is a virtually impossible task. Moreover, it is difficult to ascertain the combined roles of individual chemical constituents (e.g., synergetic effects between chemical species and transformation of chemicals through complex cellular mechanisms) in triggering adverse health effects. Thus, determination of source-specific toxicity may present a more efficient alternative means to elucidate the effects of individual PM2.5 rather than chemical component-specific toxicity measurements. The number of toxicity tests for hundreds or thousands of chemical components in PM2.5 (chemical component-specific toxicity test) can be reduced to the smaller particle groups that are major distinct sources for fine particles (source-specific toxicity test) and a range of biological responses to source-specific aerosols under comparable conditions (PM generation, collection, exposure and biological systems) can be assessed. Typically, source apportionment studies are effectively used to determine the major sources for ambient PM2.5 through measurement of their chemical components10,11. Source apportionment results can be combined with source-specific toxicity data to evaluate overall toxicity of ambient PM2.5.

The purpose of this study is to assess variability in toxicities of fine particles produced from various combustion sources and non-combustion sources which are known major sources of PM2.5 (Fig. 1). The combustion sources include diesel engine, gasoline engine, biomass burning and coal combustion. The non-combustion sources include road dust, sea spray aerosols, ammonium sulfate, ammonium nitrate, and secondary organic aerosols (SOA) produced from the photo-oxidation of toluene, 1,3,5-trimethylbenzene (TMB), isoprene, and α-pinene in the presence of NOx under natural sunlight. To determine toxicity of various particles, multiple biological and chemical endpoints (oxidative potential (OP), cell viability, genotoxicity (based on mutagenicity and DNA damage), oxidative stress and inflammatory response) using human airway cell lines, animal ovary cell lines and Salmonella strains with preexisting mutations were determined. This method facilitated the direct comparison of various endpoints linked to health effect on respiratory system with minimization of differences in exposure and biological systems. It is believed that the developed toxicity score accounts for differential toxicities of various aerosols linked to adverse health effects on respiratory system. The tested method assigned priority to PM2.5 sources potentially harmful to human respiratory system. However, the dominant mortality also came from cardiovascular diseases12. Further incorporation of toxicological data which are related to health effects on cardiovascular system could improve the toxicity score database. The multiple endpoints were integrated to derive toxicity scores for particles originating from different sources. The database for toxicity could be used to better understand health effects caused by different fine particle types of ambient PM2.5. Chemical characterization of the particles was additionally conducted to relate their major chemical components to the toxicity score for source-specific aerosols.

Figure 1.

Figure 1

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Experimental design for generation, physical and chemical characterization, and toxicity tests for various primary and secondary aerosols from different sources.

Methods

Generation of fine particles from various sources

Exhaust particles were produced from a heavy duty diesel engine (DL08S, 7640 cc, 1500 rpm, Doosan Infracore, Korea), light duty diesel engine (DL08S, 2740 cc, 1500 rpm, Isuzu motors, Japan), diesel generator engine (192FC, 498 cc, 3000 rpm, Hi-earns, China) and gasoline generator engine (GXH50, 50 cc, 5500 rpm, Honda, Japan). Table S7 summarizes the specifications of the diesel and gasoline engines used in this study. The engine exhaust particles were diluted (1:100) by an aerosol diluter (3302A, TSI Inc., USA) and sampled into PM2.5 filters for determination of chemical composition and toxicity, and real-time aerosol instruments were used to assess the number size distribution and mass concentration, as shown in Fig. 1.

A biomass burning chamber mainly consisting of a combustion chamber (0.54 m3), primary dilution chamber (3.75 m3) and secondary dilution chamber (0.04 m3), was employed for generation of rice straw and pine stem burning particles (Fig. 1). Clean air was supplied into the combustion chamber using a mass flow controller. About 25 g of biomass was loaded on the grid of the combustion stove and ignited using a propane torch. Rice straw and pine stems for biomass were collected from rural and forest areas in Korea. The smoke was drawn into a primary (3.75 m3) dilution chamber (1:1), followed by a secondary dilution chamber (0.04 m3) (1:10). Several outlets were used for PM2.5 sampling on filters for chemical and toxicity analyses and for real-time measurements of number size distribution and mass concentration with further dilution (1:100).

A bench scale high-temperature furnace (HTF55342C, Thermo Electron Corp., USA) with a quartz tube was employed to produce coal combustion particles (Fig. 1). Bituminous coal used by coal power plants (Korea South Power Co. Ltd., Hadong, Korea) was pulverized and sieved through a 200-mesh screen (< 75 µm). Pulverized bituminous coal was fed into a quartz tube (0.00327 m3) using a solid aerosol generator (SAG 410, Topas GmbH, Germany) at a feeding rate of 3.5 g/h. Several burning temperature conditions (550, 900, and 1100 °C) were used to generate coal combustion particles. Next, particles from the furnace were diluted with clean air and introduced into filters for determination of chemical composition and toxicity, and real-time aerosol instruments were used for determination of number size distribution and mass concentration. Prior to real-time measurements, samples were further diluted (1:100) using an aerosol diluter.

Road dust was collected from the shoulder of the roadside and walkway in a tunnel near a junction area in Korea (urban Gwangju). All collected samples were dried in a desiccator for 24 h. A 12-mesh sieve (< 1.7 mm) was used to remove large particles, and the dust further sieved through a 400-mesh screen (< 38 µm) to collect fine dust samples. Sieved dust was aerosolized using a solid aerosol generator under identical conditions with an input pressure of 15 psi and feeding rate of 6 g/h, as shown in Fig. 1. Aerosolized dust was subsequently introduced into the PM2.5 filter sampling system (Fig. 1). Prior to real-time measurements, aerosols were diluted (1:100) using an aerosol diluter. Carbon black powder (Cabot Corp., USA), Arizona dust13 and Mongolian dust collected from roadsides in urban Ulaanbaatar were additionally re-suspended using the above method.

Sea spray aerosols were generated from natural seawater sampled from the South sea of Korea using two system types, specifically, a marine aerosol reference tank (MART) (Marine Research Systems, USA) and a prototype bubble bursting chamber (Fig. 1). Ammonium sulfate (Sigma-Aldrich, USA) and ammonium nitrate (Sigma-Aldrich, USA) in deionized water solution were used to produce ammonium sulfate and ammonium nitrate particles with the aid of an atomizer, as shown in Fig. 1. Particles were dried using a series of diffusion driers before sampling into filters or introducing into real-time aerosol instruments.

Secondary organic aerosols (SOA) were produced under natural sunlight using University of Florida Atmospheric PHotochemical Outdoor Reactor (UF-APHOR) dual chambers (52 m3 for each) located on the roof of the Black Hall at the University of Florida (Fig. 1). Before sunrise, hydrocarbon was inserted into the chamber with a U-shaped injector. For photooxidation of toluene, HONO produced from the reaction of 0.1 M NaNO2 and 10% w/w H2SO4 aqueous solution were introduced into the chamber as a source of OH radicals. The initial mixing ratio of HONO was estimated based on the difference in NO2 concentrations with and without the base denuder (1% Na2CO3 + 1% glucose)14. Particle size distribution and concentrations of chamber-generated SOA were monitored with the aid of a scanning mobility particle sizer (SMPS) and calculated to mass concentrations using SOA density (1.3 g/cm3 for α-pinene SOA and 1.4 g/cm3 for other three types of SOA)15–17. The mass concentration of organic carbon (OC) was additionally monitored using a semi-continuous Organic carbon/Element carbon (OC/EC) aerosol analyzer. Organic matter (OM) concentration was determined by multiplying OC by the [OM]/[OC] ratio, which was calculated as 1.6 for α-pinene and 2.0 for the other three SOA types18. The chamber experiment conditions used to produce various organic aerosols and SOA yields are summarized in Table S2. Generated SOAs were collected using a Particle-Into-Liquid sampler (PILS) (Applikon, Netherlands) and toxicities were examined via chemical and biological assays.

Physical and chemical characterization of fine particles

Number size distribution of fine particles from various sources was measured with a scanning mobility particle sizer (SMPS) (3081 DMA and 3022A CPC, TSI Inc., USA) (14 to 660 nm), NanoScan SMPS (3910, TSI Inc., USA) (10 to 420 nm), and optical particle sizer (OPS) (3330, TSI Inc., USA) (0.3 to 10 μm). PM2.5 mass concentration was assessed with a Dust Trak instrument (DRX, TSI Inc., USA). For offline chemical analysis, PM2.5 filter samples (Zefluor filter for ions and elements, and quartz filter for carbonaceous species) were collected from various sources in laboratory and urban (Gwangju), rural (Jangseoung), roadside (Gwangju), and industrial (Gwangyang) sites in Korea.

Ions, elements, and carbonaceous species (OC and EC) in PM2.5 filter samples were determined via ion chromatography (IC) (850 Professional IC, Metrohm, Switzerland), inductively coupled plasma-mass spectrometry (ICP-MS) (7500ce, Agilent, USA), and an OC-EC carbon analyzer (5L, Sunset laboratory Inc., USA), respectively. A Zefluor filter was used for ion and element analyses. The filter was equilibrated at a temperature of 21 ± 2 °C and relative humidity of 35 ± 5% for 24 h before and after sampling. Triplicate pre- and post-weighing was performed for each filter. After sampling, the Zefluor filter was cut into two equal parts for analyses of ions and elements. One part of the filter was extracted with 20 mL deionized water under 1 h ultra-sonication and 3 h shaking. The water extracts were subsequently passed through a polytetrafluoroethylene (PTFE) syringe filter, and 8 water-soluble ions (SO42−, NO3−, Cl−, NH4+, Na+, K+, Mg2+, and Ca2+) analyzed via IC. Recovery was determined as 87.5–103.5% using standard samples. The second portion of the sampled filter was digested in PTFE (polytetrafluoroethylene) vessels by adding HNO3 and HCl (3:1) in a closed microwave system. The digested solution was concentrated to 0.5 mL in a heating block and treated with 2% HNO3 up to a volume of 10 mL. The solution was subsequently filtered using a PTFE syringe filter and 14 elements (Al, As, Ba, Cd, Co, Cu, Fe, Mn, Ni, Pb, Sr, Ti, V, and Zn) were analyzed via ICP-MS. Recovery was determined as 87.8–118.8% using standard samples. A quartz filter (Pall corporation., USA) was applied for analysis of carbonaceous species. The filter was prebaked at 450 °C for 5 h before sampling and stored in a freezer at −20 °C prior to and after sampling. OC and EC were measured with the OC-EC analyzer employing the thermal-optical transmittance (TOT) method19 based on the National Institute for Occupational Safety and Health (NIOSH) 5040 temperature protocol20.

For analysis of organic compounds for biomass burning particles, PM2.5 samples were extracted with dichloromethane (DCM) followed by acetone using a Soxhlet extractor. The solvent extract was subsequently reduced to a volume of 250 μL with a rotary evaporator and nitrogen blowdown. Then, the extract was methylated using diazomethane (1-methyl-3-nitro-1-nitrosoguanidine, MNNG). It was reacted with silylation reagent to derivatize levoglucosan to trimethylsilyl derivatives (TMS-derivatives). Eight classes of organic compounds (n-alkanes, hopanes and steranes, PAHs, n-alkanoic acids, benzoic acid, dicarboxylic acids, resin acid and levoglucosan) and 122 organic species were analyzed using GC-MS (7890A GC, 5975C MSD, Agilent Technologies, USA). For organic analyses of coal combustion, diesel and gasoline engine exhaust particles, PM2.5 samples were extracted using the mixture of DCM and methanol (MeOH) (3:1 by volume) with 1 h sonication. The extract was reduced to a volume of 500 μL with a Turbo Vap concentrator (Turbo Vap II, Caliper Life Sciences, USA) subjected to a gentle stream of nitrogen (99.9%). Six classes of organic compounds (n-alkanes, hopanes, PAHs, n-alkanoic acids, dicarboxylic acids, and levoglucosan (61 organic species)) for coal combustion particles and four classes of organic compounds (n-alkanes, PAHs, n-alkanoic acids, and levoglucosan (49 organic species)) for diesel and gasoline engine exhaust particles were analyzed via GC-MS (7890A GC, 5975C MSD, Agilent Technologies, USA).

Biological and chemical responses of fine particles

For assessment of particle toxicity, various chemical and biological assays were conducted. PM2.5 samples on glass fiber filters and PTFE filters (Pall corporation, USA) were extracted using DI water and dimethylsulfoxide (DMSO) (Sigma Aldrich, USA) at room temperature and dichloromethane (DCM) (Samchun chemicals, Korea) at 37 °C. For the chemical assay, OP (used to estimate the capability of PM to generate reactive oxygen species (ROS)) was measured. ROS are reported to cause oxidative stress followed by inflammation and cell death21,22. The OP was measured using two different techniques: Dithiothreitol (DTT) chemical assay (OP_DTT)23,24 and Electron Spin Resonance spectrometry (OP_ESR)25,26. OP_DTT evaluates the reaction of ROS via the formation of a DTT-disulfide as a result of electron transfer from DTT to ROS. DTT is commonly used as a chemical surrogate for cellular reducing agents (NADH and NADPH) in order to mimic in vivo interactions of PM with biological oxidants. On the other hand, OP_ESR measures the ability of PM to generate OH radicals via the Fenton oxidation in the presence of H2O2 (hydrogen peroxide) and 5,5-dimethyl-1-pyrroline-N-oxide (DMPO) as a spin trap agent. OP activities measured using both assays were employed to evaluate the ROS generation capability of PM samples.

For the biological assay, human airway epithelial (A549, H292, BEAS-2B and SAEC) and Chinese hamster ovary (CHO-K1) cell lines were used to assess in vitro cell toxicity. A variety of biological responses (cell viability, genotoxicity (mutagenicity and DNA damage), oxidative stress, and inflammation) that facilitate identification of the specific PM triggering ROS and inflammatory responses leading to oxidative stress, genotoxicity and cell death were evaluated for source-specific aerosols27–29. Neutral red uptake (NRU) (Sigma Aldrich, USA)30 and water-soluble tetrazolium salt (WST-1) assays (Takara, Japan) were utilized to determine cell viability in the presence of PM2.5. Ames test31 and the Single Cell Gel Electrophoresis (SCGE) Comet assay32 were conducted to determine mutagenicity and DNA damage of cells induced by PM, respectively. Levels of cellular reactive oxygen species (ROS) causing oxidative stress in cells were measured via the DCFDA (2′,7′-dichlorofluorescein diacetate) assay (Abcam, UK)33. Measurements of IL-6 and IL-8 (Abcam, UK) were obtained for determining the expression of genes related to the inflammatory response34. The initial PM2.5 extracts were diluted 1–150 μg/mL for NRU, 10–500 μg/mL for WST-1, 0.1–1,000 μg/mL for the Ames test, 62.5–500 μg/mL for Comet, 15–150 μg/mL for DCFDA, and 2–15 μg/mL for the IL-6 and IL-8 assays while undiluted extracts were used for OP assays. Table S8 and Supplementary Information provides details of the chemical and biological assays performed in this study. The biological effects of PM are not comparable among different studies owing to distinct exposure concentrations, biological models, endpoints, and PM generation methods35. Here, we employed similar exposure and cell conditions and identical endpoints for various aerosols within the same batch to obtain comparable toxicity data for PM2.5 from different sources. However, non-linear concentration-response functions for various endpoints and different exposure concentrations will limit the ability to use toxicological data to predict risks in human populations. Experimental design for generation, physical and chemical characterization, and toxicity tests for various primary and secondary aerosols from different sources is illustrated in Fig. 1.

Statistical information

Statistical analysis of all toxicity data performed using SPSS ver. 21.0 (IBM SPSS, USA). Differences between groups were assessed with Tukey’s post-hoc test following one-way analysis of variance (ANOVA). Statistical significance was accepted at p < 0.05.

Results

For source-specific aerosols, Fig. 2 illustrates multiple chemical and biological responses (OP, cell viability, genotoxicity and DNA damage, oxidative stress, and inflammatory response). The toxicological values employed were OP activity normalized by PM2.5 mass for OP_DTT using dithiothreitol (DTT) assay and OP-ESR using electron spin resonance (ESR), half-maximal effective concentration (EC50) for cell viability, specific activity (number of revertant colonies per PM2.5 mass) for mutagenicity, significant concentration-dependent induction factor (summation of fold change relative to control divided by dose concentration within the concentration range showing a significant dose-response relationship) for DNA damage, relative fluorescence intensity (fold change relative to control for specific dose concentration) for oxidative stress, and relative maximum cytokine production (maximum fold change relative to control divided by dose concentration from the dose-response curve) for inflammatory response. The dose concentrations among sources were in a similar range for each assay except OP. For each endpoint, relative toxicological values of source-specific aerosols were plotted as sphere size with standard deviations. The highest value among the different aerosol types for each response is taken as 100% (maximum size of the sphere). All measurements of endpoints for source-specific aerosols, except SOAs, were conducted under similar conditions to facilitate direct comparison of the contributions of the different particles to biological and chemical responses.

Figure 2.

Figure 2

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Multiple chemical and biological responses (oxidative potential, cell viability, genotoxicity (based on mutagenicity and DNA damage), oxidative stress and inflammatory response) for source-specific aerosols. The relative magnitude is plotted as the size of sphere with standard deviation, and the highest value among different aerosol types for each response is taken as 100% (maximum sphere size). aCoal combustion temperature. bEngine displacement.

Our results disclosed higher toxicity of combustion than non-combustion aerosols. Among the combustion aerosols, diesel engine exhaust particles (engine displacement of 2800 cc) were identified as the most toxic based on chemical and biological responses. Further analyses are required to confirm the statistical significance of this result as discussed in a toxicity score calculation section. In particular, genotoxicity (mutagenicity) and OP_DTT of diesel engine exhaust particles were significantly higher than those for other aerosol types. The mutagenic effects of soot particles are suggested to be associated with the organic components (e.g., PAH) generating reactive oxygen species (ROS) that are able to break DNA strands36. Polar or quinone fractions of PAH in diesel engine exhaust particles are reported to play an important role in the increased toxic response37. OP_DTT is sensitive to the amount of quinone, an oxidation product of PAHs and redox recycling agent in particles25,38. The mutagenicity of particles produced from smaller diesel engines (engine displacement of 498 cc) that emit lower levels of organic components was relatively low compared to particles from larger engines (2800 cc). A more in-depth explanation of chemical components is provided in a later section. Gasoline engine exhaust particles also showed comparable or lower toxicity relative to diesel engine exhaust particles based on various endpoints.

Both rice straw and pine stem burning particles (popular biomasses used in East Asia) showed significant toxicity in terms of effects on cell viability (NRU and WST-1) comparable to that of diesel engine exhaust particles. Pine stem burning particles were associated with higher inflammatory responses than rice straw burning particles. However, OP_ESR and OP_DTT were higher for rice straw particles. OP_DTT and OP_ESR is reported to be correlated well with OC and trace elements (Cu and Zn), respectively25,26. Rice straw particles contained higher fractions of Cu, Zn, and OC than pine stem particles, explaining the higher OP values obtained. Evaluation of the OP of PM2.5 using multiple assays is essential to provide useful complementary information.

In the case of coal combustion particles (burned at 1100 °C and 550 °C), the biological responses were relatively low compared to other combustion particle types. The bituminous coal was selected because it was the most abundant type used in the world39. Also, it has been extensively used in power plants in East Asia40,41. Variability in toxicity among different coal types was found to be small42,43. Two burning temperatures were used for simulation: residential coal combustion (low temperature) and power plant coal combustion (high temperature). Coal combustion particles generated at a temperature of 550 °C showed relatively higher toxicity in many endpoints than those at 1100 °C. One potential explanation for this finding is that the higher burning temperature leads to more complete burning of pulverized coal, leading to lower emission of carbonaceous species. Our data suggest that residential coal combustion particles typically emitted at lower burning temperatures are more toxic than power plant coal combustion particles emitted at higher burning temperatures. Levels of carbonaceous species, including toxic organic compounds (such as PAH), were significantly higher in residential than industrial coal combustion particles44.

Ammonium sulfate and ammonium nitrate particles which were aerosolized and dried from their solutions showed little toxicity in almost all endpoints. Limited in vitro toxicity data on these particles are currently available. Both neutralized sulfate and nitrate particles exert little or no effects on inflammatory responses and cell viability45,46, and inclusion of strong acids (e.g., sulfuric acid or nitric acid) in particles is proposed to lead to adverse respiratory effects47,48. The acid aerosols can increase metal solubility yielding the ROS. Earlier epidemiological studies26,49–51 indicate that sulfate has detrimental effects on human health. It is possible that sulfate in the ambient atmosphere reacts with other chemical components, leading to modification of the size and chemistry of the particles, and consequent adverse health effects, in contrast to laboratory-generated sulfate particles52. However, the issue of whether sulfate exerts combined effects with other chemical components (e.g., OC and metals) emitted at the same time remains to be established45,53.

Fine particles (< 2.5 µm) from Arizona dust13 mainly consisting of mineral/soil components showed little toxicity. Additionally, low toxicity was observed for sea spray aerosols from natural seawater based on OP and cell viability analyses (data not shown). However, we observed significant toxicity of resuspended fine dust collected from the roadsides and tunnels at urban sites based on oxidative potential, cell viability, and inflammation endpoints, which may be attributable to carbonaceous species and heavy metals originating from vehicles (engine exhaust, tire wear, and brake pad). Fine particles from carbon black powder (Cabot Inc., USA) exerted a little toxicity, suggesting that carbon black itself would not be so harmful but adsorbed components, such as organics and heavy metals, are the greater contributory factors54.

SOA showed substantial toxicity in OP and inflammatory response (IL-8) assays (Fig. 2). Specifically, OP of the SOA produced from photochemical reaction of toluene and NOx under natural sunlight (i.e., toluene SOA) using the UF-APHOR chamber (outdoor smog chamber) was higher than that obtained for other types of SOA (TMB SOA, isoprene SOA, and α-pinene SOA), and was comparable to biomass (hickory wood) burning particles. Toluene is the most abundant volatile organic compound (VOC) emitted from motor vehicles (anthropogenic source)55,56 while isoprene SOA is globally the highest VOC from natural sources57. The IL-8 response of toluene SOA was higher than TMB SOA. In our study, no cell toxicity (WST-1) was detected for both aromatic and biogenic SOA. The range of exposed mass concentrations of SOA are summarized in Table S2.

As discussed in previous sections, biological and chemical responses for source-specific aerosols varied significantly among toxicological endpoints, making accurate quantification of the representative toxicity difficult. For example, chemical components related to inflammatory responses may be distinct from those associated with bacterial mutagenicity. The relative magnitude of one response would not be feasible to represent toxicity of particles. Average toxicity data obtained for different types of diesel or gasoline engines, biomasses (rice straw and pine stem) and coal combustion temperatures were used to represent diesel engine exhaust, gasoline engine exhaust, biomass burning and coal combustion particles, respectively. A Multiple Attribute Decision Making (MADM) model was applied to derive toxicity scores of source-specific aerosols from various toxicity data. The MADM is to make choice of the best alternative among a set of alternatives with multiple and conflicting attributes58. Determination of the weights of attributes is very important in the process of decision making58. There are three types of MADM models to determine the weights of attributes (subjective, objective and integrated)58–60. In this study, Correlation Coefficient and Standard Deviation (CCSD) method, which is the objective decision making model58, was employed to determine the weights of attributes (endpoints) when the outcome (toxicity score) was simultaneously influenced by multiple attributes58. In the CCSD method, the endpoint having the highest standard variation among source types had the highest weight (i.e., differential weights were given to endpoints) without any subjective judgement. As resulted from the CCSD, the highest weight was found for cell viability followed by mutagenicity, oxidative potential, inflammatory response, and oxidative stress. The derived weights were multiplied by normalized values of endpoints to determine the toxicity score of source-specific aerosols. The toxicity score was normalized up to 10 (normalized toxicity score of source i = (toxicity score of source i − minimum toxicity score)/(maximum toxicity score − minimum toxicity score) * 10). Source with the highest toxicity has the toxicity score value closest to 10. Multiple toxicological data were integrated into the toxicity score for source-specific aerosols as shown in Fig. 3.

Figure 3.

Figure 3

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Normalized toxicity scores (0 to 10) for source-specific aerosols with differential weights (CCSD) and equal weights given to endpoints.

Overall, as shown in Fig. 3, the highest toxicity score was obtained for diesel engine exhaust particles, followed by gasoline engine exhaust particles, biomass burning particles, coal combustion particles, road dust, desert dust, ammonium sulfate, and ammonium nitrate, suggesting that traffic plays the most critical role in enhancing the toxic effects of PM2.5. Toluene SOA toxicity based on OP data was comparable to biomass burning particles. In general, the concentration of quinones is negligible in SOA. Jiang et al.57 reported that alkylperoxides produced from photo-oxidation of precursor hydrocarbons were responsible for the OP_DTT response of the resulting SOA via a non-catalytic reaction mechanism. However, due to the limited number of endpoints for SOA, the SOA data were not included in the CCSD method. In addition to the CCSD method, a linear combination of normalized values of endpoints (i.e., equal weights were given to endpoints) for each source was used to determine the toxicity score (0 to 10) of source-specific aerosols. The toxicity ranking with equal weights was similar to that obtained from the CCSD method with differential weights as shown in Fig. 3. Hereafter, the toxicity score (0 to 10) derived from the CCSD method was used for further calculation.

The aging process of freshly emitted particles in the ambient atmosphere may also modulate toxicity. For instance, aged combustion particles oxidized by ozone are suggested to exacerbate lung injury and inflammation relative to non-oxidized particles36,61,62. The effects of atmospheric aging on toxicity of particles require further investigation. The toxicity score for each source could therefore be continuously improved by adding new data sets from in vitro, in vivo, and epidemiology studies. The toxicity score results should be useful for decision makers to assess contribution of PM2.5 sources to human health and to establish PM abatement policies in addition to PM2.5 mass concentration.

Typically, source contribution for ambient PM2.5 is determined from the measured chemical components using various source apportionment methods10. By combining the toxicity scores for source-specific aerosols with the mass fractions of sources in ambient PM2.5, toxicity score for ambient PM2.5 can be derived. The toxicity score for ambient PM2.5 is the summation of values obtained by multiplying toxicity scores for source-specific aerosols by mass fractions of the corresponding sources (toxicity score for ambient PM2.5 = toxicity score of source 1 * mass fraction of source 1 + toxicity score of source 2 * mass fraction of source 2 + toxicity score of source 3 * mass fraction of source 3 + toxicity score of source 4 * mass fraction of source 4+ ….). The summation assumes that source-specific toxicity scores are additive.

We measured the OP_DTT for ambient PM2.5 samples. The OP is a good indicator of the oxidizing capability of PM without cell culture and widely used for toxicity testing of ambient aerosols due to its simplicity of application29. The oxidizing capability of PM can trigger oxidative stress followed by cell death and can be considered as an additional health metric29. The daily toxicity score for ambient PM2.5 derived here was compared with the measured OP_DTT (pmol/min/µg) as shown in Fig. 4. It revealed a moderate correlation between the two values (r = 0.49, n = 34). The toxicity score or OP_DTT was found to vary significantly even with similar PM2.5 mass concentrations which was attributable to the significant differences in the contributory sources over the experimental days.

Figure 4.

Figure 4

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Comparison of daily toxicity score with mass-normalized OP_DTT (pmol/min/µg) for ambient PM2.5.

The principal component analysis (PCA) method was applied to relate chemical and biological responses of source-specific aerosols to their chemical components. Details on chemical data for source-specific aerosols are included in Methods and Tables S3, S4, S5, and S6. For PCA, 9 samples (diesel engine exhaust particles (heavy and light duty), gasoline engine exhaust particles, rice straw burning particles, pine stem burning particles, coal combustion particles burned at 550 °C and 1100 °C, dust (tunnel and roadside)) and 30 variables including chemical components (fractions in PM2.5) and biological and chemical responses (relative magnitude in each endpoint) were used. The chemical components included 8 ions (ammonium, calcium, magnesium, potassium, sodium, chloride, nitrate, and sulfate), 14 elements (Al, As, Ba, Cd, Co, Cu, Fe, Mn, Ni, Pb, Sr, Ti, V, and Zn), 2 carbonaceous species (OC and EC), and 4 OC classes (n-alkanes, PAHs, alkanoic acids, and sugars (levoglucosan only)). The biological and chemical endpoints were oxidative potential (OP_DTT and OP_ESR), cell viability (NRU (CHO), NRU (H292) and WST-1 (A549)), genotoxicity (mutagenicity and DNA damage), oxidative stress (DCFDA), and inflammatory response (IL-6 and IL-8). As shown in Fig. 5 (loading plot), almost all toxicological endpoints were grouped together with total OC, PAHs, n-alkanes and EC in principal PC1 and PC2. The sum of PC1, PC2 and PC3 explained 78% variance of all data (PC1 = 46%, PC2 = 17% and PC3 = 16%). However, biological responses can be influenced not only by chemical composition but also interaction among chemical species, bioavailability, and oxidation state. Compounding effects between chemical species cannot be fully described by correlation analysis.

Figure 5.

Figure 5

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PCA results for relation of chemical and biological responses of source-specific aerosols to their chemical components.

Discussion

Fine particles were generated from distinct combustion (diesel engine, gasoline engine, biomass burning and coal combustion) and non-combustion (road dust, sea spray aerosols, ammonium sulfate, ammonium nitrate and SOA) sources. Measured sizes, chemical components and toxicities of source-specific aerosols varied significantly among the PM2.5 source types, suggesting that even at the same mass concentration, the effects of PM2.5 on human health are significantly variable. Various biological (cell viability, genotoxicity, oxidative stress and inflammation in respiratory cells) and chemical (oxidative potential) responses to source-specific aerosols were measured and statistically integrated to derive toxicity scores using the MADM model which determines the weights of endpoints when the toxicity score is simultaneously influenced by multiple endpoints. The highest weight was found for cell viability followed by mutagenicity, oxidative potential, inflammatory response, and oxidative stress. The weights for endpoints can be further improved by adding new assays with different cells. Also, the MADM model can be modified by putting more emphasis on specific health endpoints. Diesel engine exhaust particles had the highest toxicity score followed by biomass burning, gasoline engine exhaust, and coal combustion particles. Moreover, different size distributions of aerosols from various sources resulted in variable total and lung deposition efficiencies of particles in the human respiratory system which can be used to estimate lung dose more accurately. The tested method assigned priority to PM2.5 sources potentially harmful to human respiratory system. Further incorporation of toxicological data which are related to health effects on cardiovascular system could improve the toxicity score database. In-vivo toxicological data for acute toxicity, sub/chronic toxicity, carcinogenicity, and other toxicity can also reinforce the toxicity score.

It is not straightforward to use in vitro data (cell viability, genotoxicity, inflammatory response, oxidative potential and oxidative stress) determined in this study to predict health effects (morbidity and mortality) in human populations. In general, in vitro data can be used to rank various types of particles in terms of the toxic potential including possible carcinogenicity. Each of the marker we have studied will help to understand the hazard and differential toxicity of various fine particles.

A priori knowledge of toxicity of particles produced from various sources obtained here can be linked with source apportionment and exposure level to derive a new health metric for ambient PM2.5 in future work. The toxicity potential of various types of particles should be integrated with long term or short term health effects at individual or population level to predict public health impacts with consideration of differential toxicities of fine particles.

Conclusion

The database for toxicity scores for source-specific aerosols was constructed which can be and used to better understand the complex detrimental health effects caused by different fine particle types of ambient PM2.5. By considering differential toxicities of particles although it must be continuously improved, it could be possible to provide information that is more relevant for decision makers to establish PM2.5 abatement policies rather than only focusing on PM2.5 mass concentration.

Electronic supplementary material

Supplementary information (47.9KB, docx)

Acknowledgements

This research was supported by the National Leading Research Laboratory program (NRF-2016R1A2A1A05005532) and the PM2.5 research consortium (NRF-2014M3C8A5028593 and NRF-2017M3D8A1092220), both funded by the Ministry of Science and ICT (MSIT) and the National Research Foundation (NRF) of Korea.

Author Contributions

K.P. initiated and designed the study. K.L., M.B. and J.L. measured the chemical composition of PM2.5. M.P., H.S.G., M.J., L.J.S.B., H.L., H.S. and K.H.C. conducted toxicity tests. I.K. and S.D.K. performed toxicity score derivation. Y.H.C. and S.G.P. conducted epidemiological analysis. K.P., M.P., and H.S.J. analyzed all the data and wrote the manuscript. All authors discussed and approved the content of the manuscript.

Data Availability

Data from this study are available from the corresponding author upon reasonable request.

Competing Interests

The authors declare no competing interests.

Footnotes

Publisher’s note: Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Electronic supplementary material

Supplementary information accompanies this paper at 10.1038/s41598-018-35398-0.

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Supplementary Materials

Supplementary information (47.9KB, docx)

Data Availability Statement

Data from this study are available from the corresponding author upon reasonable request.

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