Table 2.
Strains | pH | |||
---|---|---|---|---|
Initiation | First cycle | Second cycle | Third cycle | |
G. oxydans 621H | 7.38 ± 0.03 | 7.67 ± 0.10 | 7.97 ± 0.15 | 8.17 ± 0.15 |
P. putida S16 | 7.13 ± 0.06 | 7.43 ± 0.12 | 7.67 ± 0.06 | 7.95 ± 0.05 |
The experiments were carried out in the 50-mL centrifuge tube containing 10 mL of 0.9% NaCl and magnetically immobilized cells at 30 °C, 180 rpm. NaHS was added to 2 mM to initiate the reaction in each cycle and the pH was tested at the beginning and the end of each cycle