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Experimental setup. (A) Schematic representation of biological samples used in the analysis showing (i) HSP90 able to bind ATP (orange), (ii) HSP90 bound by RAD, and (iii) HSP90rr able to bind ATP, no binding of RAD. N, N-terminal domain; M, middle domain; C, C-terminal domain. (B) Flow chart of ribosome profiling analysis.
