FIG 3.

Identification of uORFs and extended coding sequences. (A) Position of the HSP100 CDS on chromosome 29; ruler shows position in kilobase pairs. (B) RNA-Seq read alignment at the HSP100 gene locus. (C) RFP read alignment at the HSP100 gene locus. (D) Enlargement of the 5′ UTR of HSP100 showing RFPs aligned to an upstream AUG codon, representing a putative uORF. (E) Zoom into the translation initiation site of HSP100 represented by RFP reads aligning 12 nucleotides upstream of the AUG start codon. The blue bar represents the start of the HSP100 CDS. (F) Possible erroneous annotation of the LinJ.30.0460 (eIF4-E4) CDS, with RFP reads aligning well upstream of the annotated AUG start codon. Annotated and experimentally determined CDSs are shown as arrows.