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. Author manuscript; available in PMC: 2019 Mar 3.
Published in final edited form as: Nature. 2018 Sep 3;561(7722):248–252. doi: 10.1038/s41586-018-0471-x

Figure 3 |. BAK1-AAA and BAK1-S612A plants are not affected in flg22-induced FLS2-BAK1 complex formation.

Figure 3 |

a, b, Western blot analysis with α-BAK1 antibodies. c, d, Co-immunoprecipitation between FLS2 and BAK1 using α-FLS2 and α-BAK1 antibodies. Two-week-old seedlings were treated with water (−) or 100 nM flg22 (+) for 10 min before protein extraction. Blots were stained with Coomassie brilliant blue (CBB) for loading control. a-d, Experiments were repeated at least three times with similar results. For blot source data, see Supplementary Figure 1.