Table 2.
Fluorescence derivatizations after hydrolysis used in HPLC and CE.
| Analyte | Derivatizing reagent | Matrices | Separation | Temperature condition (°C) | Reaction time | λex- λem (nm) | Limit of detection | Refs. |
|---|---|---|---|---|---|---|---|---|
| Hydrolysis by phospholipase C | ||||||||
| GPCho | alpha-naphthylisocyanate | Rat liver microsomes | HPLC | 85 | 2 h | 280-360 | 10 pmol | [39] |
| GPCho, GPEtn | DNS-ethanolamine phosphate from DNS-Cl | Egg | HPLC | 60-80 | 24 h | 360-420 | - | [30] |
| GPEtn | Naproxen chloride | rat brain cerebrum and cerebellum | HPLC | 80 | 15 min | 332-352 | 1 pmol | [40] |
| GPCho, GPA | 9-anthroyl chloride | Rat thymocytes | HPLC | 60 | 10 min | 360-460 | 0.1 pmol | [41] |
| PAF | 7-methoxycoumarin-3-carbonyl chloride or 7-methoxycoumarin-4- acetic acid | Human leucocytes | HPLC | - | - | - | 100 pg | [42] |
| Hydrolysis by phospholipase D | ||||||||
| GPCho | DNS-ethanolamine from DNS-Cl | Egg | HPLC | Room temperature | 2-3 h | 360-420 | - | [31] |
| Hydrolysis by alkaline phosphatase | ||||||||
| Sphingoid base 1-phosphates | OPA | Serum, cultured cells, rat tissues | HPLC | Room temperature | 20 min | 340-455 | 0.5 pmol | [43] |
| Sphingosine 1-phosphate and dihydro Sphingosine 1-phosphate | OPA | Cultured cells, plasma | HPLC | Room temperature | 20 min | 340-455 | <0.5 pmol | [44] |