Figure 2.

(a) Relationship between the initial rate of NADH oxidation and the concentration of branched P450cam at a water content (W₀) of 16.7. The reaction mixture contained 5 mM d-camphor and 80 μM NADH. (b) Effect of W₀ on the initial activities of branched P450cam (open circles) and an equimolar mixture of PdR, PdX and P450cam (closed circles). The reaction mixtures contained 0.05 μM protein, 5 mM d-camphor and 80 μM NADH. The reversed micelle solution containing PdR, PdX and P450cam was prepared by adding a mixture of PdR, PdX and P450cam to a starter reversed micelle solution (see experimental section).