Table 1.
In vitro cleavage reactions of the HIV-5’ UTR by catalytic RNAs. (a)T0.5 values were normalized to the one of the corresponding ribozyme. (b) Regression curves were fixed to a maximum cleavage value of 100%.
| RNA | Maximum cleavage (%) | T0.5 (min) | Relative T0.5(a) | sqr(R) (b) |
|---|---|---|---|---|
| HH113 | 100 ± 14.23 | 22.89 ± 9.88 | 1 | 0.8824 |
| HH113DIS | 100 ± 10.21 | 24.92 ± 7.43 | 1.08 | 0.9417 |
| HH113αDIS | 100 ± 7.99 | 25.4 ± 5.88 | 1.11 | 0.9667 |
| HP113 | 100 ± 3.78 | 16.25 ± 2.17 | 1 | 0.9863 |
| HP113DIS | 100 ± 6.15 | 32.34 ± 5.17 | 1.98 | 0.9823 |
| HP113αDIS | 100 ± 5.67 | 20.99 ± 3.75 | 1.28 | 0.9746 |
| HH159 | 100 ± 21.26 | 118.9 ± 39.54 | 1 | 0.9764 |
| HH159DIS | 100 ± 10.94 | 75.48 ± 15.15 | 0.63 | 0.986 |
| HH159αDIS | 100 ± 11.84 | 62.25 ± 14.57 | 0.52 | 0.9783 |
| HP159 | 100 ± 9.53 | 19.47 ± 6.05 | 1 | 0.9369 |
| HP159DIS | 100 ± 14 | 24.44 ± 10.08 | 1.25 | 0.8942 |
| HP159αDIS | 100 ± 9.1 | 16.61 ± 5.28 | 0.85 | 0.9322 |