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Sensorgrams of PEG immobilization on gold surfaces. (a) PEG5k(3), (b) PEG5k(1)/2k(3), and (c) PEG5k(2)/2k(4). Flow rate, 10 μL/min; running buffer, PBS (0.15 M, pH 7.4, containing 1 M NaCl); sample, 0.01 mg/mL of PEG (Mw: 5k or 2k)/PBS (0.15 M, pH 7.4, containing 1 M NaCl) solution; sample injection, 100 μL for each time point [84].
