Figure 4.

Pharmacological assays on mouse phrenic nerve-diaphragm preparations to determine the twitch response under electric indirect stimuli after incubation the preparation with 15 µg/mL Crotalus durissus terrificus (Cdt) venom pre-incubated for 30 min with the D. alata barks extract (0.05 mg/mL) using different solvents (HE, hexane; DE, dichloromethane; EAE, ethyl acetate; ME, methanol). Note that none of the extracts were able to prevent the total neuromuscular blockade promoted by Cdt venom, except DE which did not prevent the blockade but instead delayed temporarily its appearance. Each point represents the mean ± S.E.M. of the number of experiments (n) showed in the legend. *p < 0.05 compared to venom.