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. 2018 Nov 26;13(11):e0208213. doi: 10.1371/journal.pone.0208213

Correction: Metformin reveals a mitochondrial copper addiction of mesenchymal cancer cells

Sebastian Müller, Antoine Versini, Fabien Sindikubwabo, Guillaume Belthier, Supaporn Niyomchon, Julie Pannequin, Laurence Grimaud, Tatiana Cañeque, Raphaël Rodriguez
PMCID: PMC6258246  PMID: 30475897

Fig 2 is incorrect. The authors have provided a corrected version here.

Fig 2. Biguanides directly target mitochondria and promote copper oxidation.

Fig 2

(A) Schematic illustration of the labeling of Met in cells using click chemistry. (B) Fluorescence microscopy images of labeled Met (green). Cells were treated with Met and subjected to click-labeling as described in Methods. Mitochondria were detected using cytochrome c immunostaining (red) and 4',6-diamidino-2-phenylindole (DAPI) stains nuclear DNA (blue). Scale bar, 10 μm. (C) Fluorescence microscopy detection of Cu2+ in cancer cells treated as indicated for 48 h. Scale bar, 10 μm. (D) Flow cytometry analysis of mitochondrial Cu2+ in cancer cells treated as indicated for 48 h. Bar chart represents an average of three independent experiments. (E) Flow cytometry analysis of mitochondrial Cu+ in cancer cells treated as indicated for 48 h. Bar chart represents an average of three independent experiments. (F) Cyclic voltammetry measurements towards oxidation potentials (purple arrow) of a Cu+ solution. Data recorded in the absence (black) and presence of 2 mol equiv. metformin (blue) or 2 mol equiv. Met (red). Redox peak potentials are marked with dashed lines. (G) Flow cytometry analysis of Fe2+ in cancer cells treated as indicated for 48 h. MDA-MB-468 cells were used in Fig 2C, 2D, 2E and 2G and were treated as described in Methods. Cells were treated at the IC50 concentration at 72 h of Metformin or Metforminyn respectively, unless stated otherwise. See also S6, S7, S8, S9, S10 and S11 Figs. Bar chart represents an average of three independent experiments.

Reference

  • 1.Müller S, Versini A, Sindikubwabo F, Belthier G, Niyomchon S, Pannequin J, et al. (2018) Metformin reveals a mitochondrial copper addiction of mesenchymal cancer cells. PLoS ONE 13(11): e0206764 10.1371/journal.pone.0206764 [DOI] [PMC free article] [PubMed] [Google Scholar]

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