Table 1.

Modified/natural nucleotide incorporation in primer extension reaction using KOD mutants and Vent(exo-) DNA polymerase. ^a

DNA polymerase	dNTP	Vmax (min–1)	Km (mM)	Insertion efficiency Vmax/Km	Accuracyb
KOD1	T	1.8	0.021	88	1
	1	11	0.14	81	0.92
	2	0.10	0.26	3.8	0.043
	C	0.074	0.66	0.11	0.0013
KOD2	T	2.1	0.035	60	1
	1	4.0	0.083	48	0.80
	2	0.32	0.099	3.3	0.055
	C	0.019	0.11	0.17	0.0029
KOD3	T	2.3	0.047	49	1
	1	4.4	0.081	55	1.1
	2	0.27	0.099	2.7	0.055
	C	0.033	0.70	0.048	0.00097
KOD8	T	9.5	0.063	150	1
	1	53	0.31	170	1.1
	2	2.4	0.38	6.3	0.041
	C	0.11	1.0	0.11	0.00073
Vent(exo-)	T	0.34	0.0030	110	1
	1	0.29	0.0027	110	0.95
	2	0.049	0.0044	11	0.099
	C	0.14	1.7	0.083	0.00073

^aExperimental conditions are described in Experimental Section. ^bThe initial rate relative apparent insertion efficiency (accuracy); (V_max/K_m)_analogue/(V_max/K_m)_TTP and (V_max/K_m)_dCTP/(V_max/K_m)_TTP.