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. 2011 Mar 11;16(3):2365–2374. doi: 10.3390/molecules16032365

Figure 3.

Figure 3

Inhibitory effect of AC-PACs on the differentiation of human pre-osteoclasts. Cells were treated with various concentrations of AC-PACs and cultivated in the presence of both M-CSF and RANKL. (A) TRAP staining was performed to evidence multinuclear cells. 1) Cells treated with M-CSF only (negative control); 2) Cells treated with M-CSF + RANKL (positive control); 3) Cells treated with M-CSF + RANKL + 10 µg/mL AC-PACs; 4) Cells treated with M-CSF + RANKL + 25 µg/mL AC-PACs; 5) Cells treated with M-CSF + RANKL + 50 µg/mL AC-PACs. (B) % of multinuclear TRAP-stained cells: Control – 100%; 10µg/mLAC-PAC - 62 ± 7%; 25µg/mL AC-PAC – 16± 7%; 50µg/mL AC-PAC – 5 ± 1%. * p < 0.05, ** p < 0.01.