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Agarose gel (1.5%) showing PCR amplified product from positive controls (recombinant plasmids containing full length VP2 gene of CPV-2 vaccine and field strains). Lane M: 100 bp plus DNA ladder (Thermo Scientific, USA), Lane 1: PCR amplified product with CPV-2 vaccine (CaniShot® DHPPL), Lane 2: PCR amplified product with CPV-2 field strain (Isolate KN-1/2015; NCBI GenBank Accession number: KX425922), Lane 3: PCR amplification from healthy unvaccinated dog, Lane4: Non-template control
