Table 1.
Primers used for ARMS-PCR differentiating vaccine and field strains showing primer sequence, position within the VP2 gene, amplicon size for vaccine and field strains (deliberate mismatch within the inner specific CPV-2 primer of vaccine and field strains has been underlined and in bold)
| Primer code | Sequence (5′–3′) | Primer position within VP2 gene | Amplicon size (in bp) |
|---|---|---|---|
| CPV-OF | ATGAGTGATGGAGCAGTTCAACC | 1–23 | 721 |
| CPV-OR | CATCATCTGGATCTGTACCATG | 721–699 | |
| CPV-IF (vac) | AGAAGAGTGGTTGTGAATAGTA | 238–260 | 721 + 483 |
| CPV-IR (field) | CGTTAACTGCAGTTTTATCGAA | 282–260 | 721 + 280 |