Table 1.
Primer name | Forward primer | Reverse primer |
---|---|---|
aUL83a | GCGAGACCGTGGAACTGC | TTGCCCTGGATGCGATACTG |
aGAPDHa | GACCCCTTCATTGACCTCAACTA | TCCTGGAAGATGGTGATGGG |
IE1 outerb | CTGTATGTGACCCATGTGCTT | GGGGCAACTTCCTCTATCTCA |
IE1 innerb | TCAGCCTTCCCTAAGACCAC | ACATGGTCATCATACAAGCG |
RNA-LUNAb | CGCGGTTATTATCAACGTCT | GCTCGTCATCCCCTCGTG |
RNA-US28b | ACTGCCTGTTTCTACGTGGCTA | ATAAAGACAAGCACGACCGCTA |
RNA-vIL10b | ATTGCAAGATCTCCGCGTCACC | CCGCAACCTAACAGAGGGCATT |
RNA-UL44 | CGGCGGTGGCGGCAAGAAG | AGAATCCTCGCTGTCGCTCTCCTC |
qIE1 | GCCTTCCCTAAGACCACCAAT | ATTTTCTGGGCATAAGCCATAATC |
qIE2 | ATGGTTTTGCAGGCTTTGATG | ACCTGCCCTTCACGATTCC |
qUL44 | GTCGGGAACAGCGGCAAGTC | CGCCACCTCCTCCCAGACC |
qUL54 | CCGTGTCACCTAACGCCGCTATC | CGCCGACGCTGCTACTACTGTTAC |
qUL94 | TGACGCAGTCGCTCATCCACAAC | CCAGGTCCCACACATCGGTCTTG |
qUL99 | GGGTCGCCAGGTGTCTCTACG | GCTGCCGCTACTATTGTCGTTTCC |
GAPDHc | TCTCTGCTCCTCCTGTTCGAC | GACAAGCTTCCCGTTCTCAG |
The primers are used for determination of viral genome copies and are the same as that used in our previous report (Fu et al., 2015).
The primers are the same as that used in our previous report (Duan et al., 2014).
The primers are used in the assay of RT-PCR and qPCR.