Skip to main content
. 2011 Apr 29;16(5):3618–3635. doi: 10.3390/molecules16053618

Figure 3.

Figure 3

(a). EM-ISH with an antisense probe for rat GH mRNA reveals its localization on the rough endoplasmic reticulum (RER) as osmium black signals. Subsequent immunohistochemical staining using anti-rat GH antibody and 20 nm protein A colloidal gold particles (arrows) identifies rat GH mainly on the secretory granules and also in the cisternae of the RER (arrow heads) (bar = 200 nm, [6]); (b). Immunohistochemical control experiments reveal no positive reactions of protein A colloidal gold particles on the secretory granules, whereas positive signals for GH mRNA are identified as osmium black on the polysomes of the RER (bar = 200 nm, [6]).