Table 3.
Media | Immobilization method | Enzyme | Advantage and disadvantage | Ref. |
---|---|---|---|---|
PDMS/Glass | Place PVDF membrane that adsorbs enzymes | Trypsin | Easy preparation Less efficiency Possibility of leakage at higher flow rate |
[56] |
Glass | Covalent cross-linking with Nylon membrane formed at liquid-liquid interface (glutaraldehyde) | Horseradish peroxidase | Integration of membrane permeation and enzyme reaction Preparation of multiple membrane Complicated preparation method Unstable membrane at higher flow rat |
[57] |
PTFE | Enzyme-embedded membrane formation using glutaraldehyde/ paraformaldehyde | α-Chimotrypsin Trypsin α-Aminoacylase Other various enzymes |
Easy preparation Durable (>40days) Applicable in organic solvents Almost all enzymes can be immobilized by adding poly-Lys |
[58,59,60] |