Skip to main content
PMC home page
Molecules logoLink to Molecules
. 2011 Jun 7;16(6):4681–4694. doi: 10.3390/molecules16064681

Synthetic Routes and Biological Evaluation of Largazole and Its Analogues as Potent Histone Deacetylase Inhibitors

Shang Li 1,2,3, Hequan Yao 1, Jinyi Xu 1,*, Sheng Jiang 2,3,*
PMCID: PMC6264525  PMID: 21654576

Abstract

Natural products with interesting biological properties and structural diversity have often served as valuable lead drug candidates for the treatment of various human diseases. Largazole, isolated from the marine cyanobacterium Symploca sp. has exhibited potent inhibitory activity against many cancer cell lines. Besides, it shows remarkable selectivity between transformed and nontransformed cells, which is the main disadvantage of other antitumor natural products such as paclitaxel and actinomycin D. Due to its potential as a potent and selective anticancer drug candidate, a great deal of attention has been focused on largazole and its analogues. It is the aim of this review to highlight synthetic aspects of largazole and its analogues as well as their preliminary structure–activity relationship studies.

Keywords: largazole, histone deacetylase inhibitor, natural products, total synthesis, biological evaluation

1. Introduction

Chromatin template activities, including DNA transcription, replication, and repair, are regulated by a variety of posttranslational modifications, among which histone acetylation plays a prominent role. Histone deacetylase inhibitors (HDACIs) have long been used in psychiatry and neurology as mood stabilizers and antiepileptics. More recently, HDACIs are being studied as targeted therapies for the treatment of neurodegenerative diseases [1]. Largazole (1, Figure 1) is a natural macrocyclic depsipeptide isolated from a Floridian marine cyanobacterium Symploca sp. by Luesch and co-workers [2]. The growth-inhibitory activity of largazole is shown considerably higher for cancer cell lines (GI50 = 7.7 nm) than for the corresponding nontransformed cells (GI50 = 122 nm). It has shown promising selective biological activity for differential growth inhibition in a number of transformed and nontransformed human and murine derived cell lines. The remarkable selectivity of largazole against cancer cells has prompted research on its mode of action and its importance as a potential cancer chemotherapeutic agent, and several research groups have completed its total synthesis. This review focuses on recently developed novel synthetic routes and their applications in the development of conformational constrained analogues of largazole, as well as biological evaluation and the preliminary structure–activity relationships of largazole and its analogues are also discussed.

Figure 1.

Figure 1

Open in a new tab

Synthesis strategies and key building fragments.

2. Synthetic Routes and Biological Evaluation of Largazole and Its Analogues

Largazole is a densely functionalized macrocyclic depsipeptide consisting of an α-methylcysteine-derived thiazoline coupled to a thiazole embedded within a 16-member macrocycle and a caprylic acid-derived thioester, which is rarely found in natural products. The retrosynthetic analysis of largazole shows that two cyclization sites A and B exist in its structure (Figure 1). To synthesize largazole, most research groups have used the following three key building blocks: valine (2), β-hydroxy ester 3a, and thiazolylthiazoline 4.

Luesch and co-workers were not only the first to isolate the natural largazole, but also the first to complete the total synthesis of largazole in collaboration with Hong and co-workers [3]. The condensation of compound 5 with (R)-2-methylcysteine methyl ester provided the key building block 4a. Removal of the Boc group, followed by coupling of amine 4b with 6, which was prepared by a Nagao aldol reaction [4,5], Yamaguchi esterification or DCC-coupling reaction, with N-Boc-L-valine (2a) provided 8. Subsequent hydrolysis and deprotection provided a precursor to the 16-member cyclic depsipeptide core. The macrocyclization of the precursor using HATU–HOAt (HATU, HOAt, i-Pr2NEt) proceeded smoothly to give 9 in 64% yield (in three steps). Thioester 10 was prepared by coupling the thioacid with 4-bromo-1-butene. The olefin cross-metathesis reaction of thioester 10 with the macrocycle 9 in the presence of 50 mol% of Grubbs’ second-generation catalyst in refluxing toluene provided largazole in 41% yield, a better result compared with that obtained in the presence of Hoveyda-generation catalyst (Scheme 1).

Scheme 1.

Scheme 1

Open in a new tab

Synthetic route of largazole developed by Luesch and co-workers in collaboration with Hong and co-workers.

By using this strategy, Luesch and his collaborators also synthesized several key analogues [6]. The acetyl analogue 12 was prepared by the olefin cross-metathesis reaction of the macrocycle 9 with thioacetic acid S-but-3-enyl ester (50 mol % of Grubbs’ second-generation catalyst, toluene, reflux, 50%). The same procedure was used to synthesize 13, except 1-triisopropylsiyloxyl-3-butene was used instead of thioacetic acid S-but-3-enyl ester, followed by deprotection. In addition, the aminolysis of 1 provided the thiol analogue 14 in 70%–80% yield (Scheme 2).

Scheme 2.

Scheme 2

Open in a new tab

Synthesis of largazole analogues 12–14.

The olefin cross-metathesis reaction of the macrocycle 9 with the products obtained from coupling the thioester with 3-bromo-1-propene, 5-bromo-1-pentene, and 6-bromo-1-hexene in the presence of Grubbs’ second-generation catalyst provided 15, 16, and 17, respectively (Scheme 3).

Scheme 3.

Scheme 3

Open in a new tab

Synthesis of largazole analogues 15–17.

Alanine analogue 20 and C17-epimer 25 were prepared by replacing the valine and (3S)-hydroxy-carboxylic acid with the alanine and (3R)-hydroxycarboxylic acid, respectively (Scheme 4).

Scheme 4.

Scheme 4

Open in a new tab

Synthesis of largazole analogues 20 and 25.

Luesch and co-workers showed that histone deacetylase (HDAC) is the cellular target for largazole’s antiproliferative activity. Largazole is a prodrug, and 14 is the reactive metabolite. Largazole, its thiol analogue 14, and its acetyl analogue 12 all exhibited similar cellular and antiproliferative activities against HDACs. However, the hydroxyl analogue 13 and the macrocycle 9 did not show any inhibitory activity. This result suggests that the thiol group is indispensable for both activities. Alanine analogue 20 showed approximately a 2- to 3-fold decrease in both activities compared with 1. The five- and six-atom linkers in 16 and 17, respectively, reduced the cell growth and HDAC inhibitory activity by several orders of magnitude, whereas 15 with the shorter chain showed no activity. The C17-epimer 25 lacked significant HDAC inhibitory activity compared with 1, suggesting that the four-atom linker between the macrocycle and the octanoyl group in the side chain and the (S)-configuration at the C17-position are all critical to the potent HDAC inhibitory activity of 1. The valine residue in the macrocycle can be replaced with the alanine without compromising its activity to a large extent.

Cramer and co-workers [7] have developed a short route for the synthesis of largazole (1), in which largazole was obtained in 19% overall yield. The β-hydroxy ester fragment 3c, which was obtained by an enzymatic resolution of racemic alcohol, was subjected to the esterification with Fmoc-L-valine followed by the deprotection of the Fmoc group to provide 26. Nitrile 5 was allowed to react with (R)-α-methylcysteine hydrochloride under mild aqueous conditions to provide the thiazoline acid 4a in an excellent yield. The intermediate 27 was prepared by coupling 4a with the amine 26 in the presence of HATU. The deprotection of 27 provided the lactam 9 in 77%–89% yield in a dilute solution of HATU and Hüing’s base. In the optimization of the cross-metathesis reaction, Cramer and co-worker found a yield increased from 44% to 75% when the p-nitro-substituted catalyst was used instead of the Hoveyda-Grubbs second-generation catalyst with the catalyst loading of 15% in CH2Cl2 at 80–100 °C. Thus, Largazole and its analogues 14, 16, 17, 28, 29, and 30 were prepared in comparable yields (37%–92%) under the optimized conditions (Scheme 5).

Scheme 5.

Scheme 5

Open in a new tab

Synthetic route of largazole and its analogues developed by Cramer and co-workers.

The findings of the antiproliferative activity of 1 and its analogues showed that the octanoic thioester of 1 acts as a protecting group and that the free thiol 14 has slightly lower potency but significantly higher specificity compared with the thioester of 1. The intermediate 9 and the analogue 28 showed no growth inhibitory activity. The replacement of the thioester with an ester group in the compound 30 led to a complete loss of activity. Thioester derivatives 16 and 17 showed no activity at all. It suggests the importance of positioning thio functionality at the right distance from the cyclic core and the necessity of the thiobutenyl group for its antiproliferative activity.

Ye and co-workers [8] have accomplished the total synthesis of 1 in 5.8% overall yield from 3-[(tert-butyldimethylsilyl) oxy] propane 33. The allylic alcohol fragment 37 was prepared by a series of reactions: oxidation of alcohol 33, Wittig reaction, reduction of ester 34, oxidation of 34 to give the enal 35, and then reaction with Nagao’s chiral N-acetylthiazolidine-2-thione 36 [4,5]. The displacement of the auxiliary of the alcohol 37, followed by the esterification of 39, provided the intermediate 40, which was converted to its corresponding disulfide 41. The fragment 4a was prepared according to Pattenden’s procedure (Et3N, MeOH, 50 °C) [9]. Hydrolysis of the methyl ester 4a provided the acid, and it was coupled with the product obtained from the removal of the Fmoc group of the disulfide 41 to give the linear depsipeptide 42. The deprotection of both Boc and TMSE ester groups of the depsipeptide 42 followed by treatment with HATU provided the cyclodepsipeptide 43. Largazole was prepared by the reductive cleavage of the disulfide bond in the cyclodepsipeptide 43 followed by the reaction with octanoyl chloride (Scheme 6).

Scheme 6.

Scheme 6

Open in a new tab

Synthetic route of largazole developed by Ye and co-workers.

Ghosh and Kulkarni have also completed an enantioselective total synthesis of largazole [10]. The condensation of the thiazole acid 48 and protected (R)-2-methylcysteine (49) gave 50, and then treatment of 50 according to Kelly’s procedure [11] followed by reduction and protection provided the thiazole ester 4a. The requisite thioester was obtained by reaction of 3-butene-1-thiol (44) with octanoyl chloride. A cross-metathesis of thioester and alcohol 3c was performed followed the same way used by Cramer and his co-workers in the presence of 3 mol % of Grubbs’ second-generation catalyst. The Yamaguchi esterification of N-Boc-valine, the deprotection of the Boc group, and the saponification of the thiazole ester 4a were performed. The coupling of the amine 46 to the acid 4a was carried out by using HATU and HOAt to prepare 51. By removing the protecting Boc and tert-butyl groups of 51, compound 1 was synthesized under dilute conditions with HATU (2 equiv.) and HOAt (2 equiv.) in the presence of diisopropylethylamine (Scheme 7).

Scheme 7.

Scheme 7

Open in a new tab

Synthetic route of largazole developed by Ghosh and Kulkarni.

Doi and co-workers [12] synthesized unit 4d using Kelly’s method, as did Ghosh and co-workers. They also carried out one-pot bisthiazoline formation using Ishihara’s method [13]. Unit 57 was prepared by the asymmetric aldol reaction of aldehyde 55 and a modified Nagao reagent, N-acetyl-thiazolidinethione 56 [14]. The subsequent amidation of 57 with the amine prepared from 4d by the removal of the Fmoc group provided a thiazoline–thiazole alcohol 58. To synthesize 59, the thiazoline–thiazole alcohol 58 was subjected to the Yamaguchi esterification of the hydroxyl group with Fmoc-Val-OH, hydrolysis of methyl ester, and then removal of the Fmoc group. The desired cyclic depsipeptide 67 was obtained by using HATU and DIPEA under high-dilution conditions. The deprotection of the trityl group of 60 provided thiol 14, and then it was allowed to react with octanoyl chloride to give 1. The analogous 12 and 61 were prepared by treating the thiol 14 with acetic anhydride and 2ʹ2-dipyridyl disulfide, respectively (Scheme 8).

Scheme 8.

Scheme 8

Open in a new tab

Synthetic route of largazole and its analogous by Doi and co-workers.

The HDAC inhibitory activity of Largazole and its analogous 12, 14, and 61 was similar, whereas the thiol 14 was slightly more potent than the S-substituted compounds, suggesting that thiol 14 is probably a real active form that is released within the cells.

Phillips [15] and co-workers synthesized the macrocycle 9 started with the Boc-protected glycine thioamide 31 and ethyl α-bromopyruvate converted to thiazolyl amide 62 by Hantzsch thiazole synthesis [16]. Dehydration of amide 62 produced the nitrile 5, and it was allowed to react with α-methylcysteine to give thiazolylthiazoline 4c under mild aqueous conditions, the same as Cramer and his co-workers. Coupling of Fmoc-Val-OH with the β-hydroxy ester 3c, which was obtained by enzymatic resolution of its corresponding racemic aldol adduct, and the subsequent removal of the Fmoc group provided amine 26. Condensation of amide 26 and 4c was accomplished with DCC and pentafluorophenol. After removal of Boc and tert-butyl groups, 27 was subjected to macrolactamization with PyAOP and DMAP in acetonitrile to provide macrocycle 9 in 50% yield. 1 was obtained in 34% yield in the presence of 20 mol% of Grubbs’ second-generation catalyst (Scheme 9).

Scheme 9.

Scheme 9

Open in a new tab

Synthetic route of largazole and some analogues developed by Phillips and co-workers.

The prepared analogues such as ester 30, ketone 64, seco-ester 65, macrocycle 9, and largazole (1) were tested for differential inhibitory activity. The results showed that only largazole preferentially acted on tumor cells. It is shown that the cellular target of largazole is HDAC and that the conformation of the depsipeptide is important for targeting HDAC, as 9, 30, 64, and 65 did not show any activity. Phillips and co-workers also confirmed the preliminary conformation of largazole.

Forsyth and Wang [17] developed a novel synthesis strategy. The Julia olefination using α,β-epoxy aldehyde 75 and thioester 74 containing a tetrazolyl sulfone moiety, which was prepared by the Mitsunobu reaction, gave alkenes. Removal of the TBS protecting group and the oxidation of the primary alcohol of 76 provided the unstable α, β-epoxy aldehyde 77. The requisite 79 was prepared from α,β-epoxy aldehyde 77 by N-heterocyclic carbene–mediated esterification with fluoren-9-ylmethanol. The azido thioester 71 was prepared from 66 by a series of reactions: amide formation, thiol substitution, and thioesterification. Treatment of the azido thioester 71 with PPh3 in acetonitrile under microwave irradiation gave the polypeptide. The acid fragment 72 was obtained by the deprotection of Boc and tert-butyl groups of the polypeptide and by an efficient Fmoc derivatization. Largazole was obtained by the Yamaguchi esterification of 79 and acid 72, followed by deprotection and macrolactamization (Scheme 10).

Scheme 10.

Scheme 10

Open in a new tab

Synthetic route of largazole developed by Forsyth and Wang.

Williams and co-workers [18] synthesized the thiazoline–thiazole subunit 4c by condensation of nitrile 5 and (R)-2-methylcysteine methyl ester hydrochloride under basic conditions (triethylamine in methanol, Scheme 3). Thiazolidinethione 81 was treated with 2-(trimethylsilyl)ethanol to prepare TSE-protected acid, and then it was allowed to couple with N-Fmoc-L-Val to prepare 82. The deprotection of the Fmoc group of 82, followed by PyBOP-mediated coupling with the thiazoline–thiazole subunit 4c provided 83. Removal of protecting Boc and TMSE groups of 83 under high dilution in the presence of HATU (2 equiv) and HOBt (2 equiv) gave the macrolactamization product 60. Removal of the protecting S-trityl group from 60 provided the thiol 14. The acylation of 14 with octanoyl chloride under standard conditions produced largazole (Scheme 11).

Scheme 11.

Scheme 11

Open in a new tab

Synthesis of largazole developed by Williams and co-workers.

During the synthesis of largazole, a wide range of analogues were obtained by Williams and co-workers [19]. According to the assay data of these analogues (Figure 2), the potency of the enantiomer of largazole decreased by almost three orders of magnitude. The C-2 epimer, the valine-to-proline substitution, and the thiazole–thiazole derivative showed intermediate potency. It shows the importance of the obligate stereochemical and conformation activity relationships between the natural product and its protein targets. The oxazoline–oxazole analogue showed a similar activity compared with largazole. It suggests that the role of the natural 3-hydroxy-7-mercaptohept4-enoic acid moiety in Largazole is significant for its activity, which can also be known in FK228 and spiruchostatin. The thiazole–pyridine substitution possessed subnanomolar activity, which was three to four times more potent than largazole [20]. It seems that the methyl substituent of the thiazoline ring is not essential for the potency of the natural product.

Figure 2.

Figure 2

Open in a new tab

The structural modification pathways of largazole.

Recently, our efforts have been focused on the total synthesis of largazole [21], we performed the solid-phase synthesis of key fragment byusing the 2-chlorotrityl chloride resin 84. The thiazoline–thiazole fragment 4a was successfully prepared by tandem deprotection–cyclodehydration and subsequent oxidation with activated manganese dioxide (Scheme 12).

Scheme 12.

Scheme 12

Open in a new tab

Synthesis of thiazoline–thiazole fragment 4a.

The primary alcohol 92 was prepared from commercially available (−)−malic acid (88). Swern oxidation of the alcohol and the Julia–Kocienski olefination followed by coupling with sulfone gave the key intermediate 94 in a favorable E/Z ratio (8/1). The selective deprotection of the primary TBS and the Mitsunobu reaction provided 95. The allylic alcohol was coupled with enantiomerically pure amino acids to prepare the intermediate 97. Removal of the Fmoc group followed by coupling with thiazoline–thiazole acid 4a gave cyclization precursor 98. The 16-member cycloamide was prepared by removal of protected group and subsequent macrolactamization (Scheme 13).

Scheme 13.

Scheme 13

Open in a new tab

Synthesis of largazole developed by our group.

Using this strategy, a small library of largazole analogs was developed. The analogues with cis- alkenes showed no activity against either human tumor cell lines or normal cell lines. Compound 104 showed slightly lower potency, but much improved selectivity for cancer cell lines. Structure-activity relationships studies suggested that the geometry of the alkene in the side chain is critical. While the largazole analogues with trans-alkene are potent for the antiproliferative effect, those with cis-alkene moieties are completely inactive. Most importantly, replacement of valine with tyrosine in largazole increased selectivity toward human cancer cells over human normal cells more than 100-fold.

3. Conclusions

The recent progresses in synthesis of largazole and its analogues are reviewed, in which several competitive synthetic routes, and its preliminary structure–activity relationships are discussed. Studies on the biological evaluation of largazole and its analogues have revealed that largazole is a pro-drug, which inhibits the growth of cells by releasing active free thiol group within the target site. A large body of investigations has provided important insight into the structural, functional, stereochemical, and conformational aspects of the largazole molecular scaffold which will constitute the basis for the further design and synthesis of extraordinarily potent HDAC inhibitors with potential therapeutic significance.

Acknowledgments

We thank the National Major Scientific and Technological Program for Drug Discovery (Grants 2009ZX09103-101), NSFC (Grants 20802078, 20972160), Shanghai University Distinguished Professor (Eastern scholars) Program (DF2009-02), Pujiang Talent Plan Project (09PJ1409200) and MOST (Grants 2009CB940900) for financial support.

Abbreviations

BOP

(1H-benzotriazol-1-yloxy) tris(dimethylamino)-phosphonium hexafluorophosphate

DCC

dicyclohexylcarbodiimide

DIAD

diisopropyl azodicarboxylate

DMAP

4-(dimethylamino)pyridine

DMF

dimethylformamide

DMSO

dimethylsulfoxide

EDC

1-ethyl-3-(3’-(dimethylamino)propyl)carbodiimide

HATU

-[(dimethylamino)-1H-1,2,3-triazolo[4,5-b]pyridin-1-ylmethylene)-N-methyl-methanaminium hexafluorophosphate N-oxide

HBTU

N-[(1H-benzotriazol-1-yl)-(dimethylamino)methylene]- N-methylmethanaminium hexafluorophosphate N-oxide

HOAt

1-hydroxy-7-azabenzotriazole

HOBt

1-hydroxybenzotriazole

TBAF

tetrabutylammonium fluoride

TEMPO

2,2,6,6-tetramethylpiperidine 1-oxyl

TFA

trifluoroacetate

THF

tetrahydrofuran

Footnotes

Sample Availability: Not available.

References and Notes

  • 1.Minucci S., Pelicci P.G. Histone deacetylase inhibitors and the promise of epigenetic (and more) treatments for cancer. Nat. Rev. Cancer. 2006;6:38–51. doi: 10.1038/nrc1779. [DOI] [PubMed] [Google Scholar]
  • 2.Taori K., Paul V.J., Luesch H. Structure and Activity of Largazole, a Potent Antiproliferative Agent from the Floridian Marine Cyanobacterium Symploca sp. J. Am. Chem. Soc. 2008;130:1806–1807. doi: 10.1021/ja7110064. [DOI] [PubMed] [Google Scholar]
  • 3.Ying Y., Taori K., Hong J., Luesch H. Total Synthesis and Molecular Target of Largazole, a Histone Deacetylase Inhibitor. J. Am. Chem. Soc. 2008;130:8455–8459. doi: 10.1021/ja8013727. [DOI] [PubMed] [Google Scholar]
  • 4.Nagao Y., Hagiwara Y., Kumagai T., Ochiai M., Inoue T., Hashimoto K., Fujita E. New C-4-chiral 1,3-thiazolidine-2-thiones: excellent chiral auxiliaries for highly diastereo-controlled aldol-type reactions of acetic acid and .alpha.,.beta.-unsaturated aldehydes. J. Org. Chem. 1986;51:2391–2393. doi: 10.1021/jo00362a047. [DOI] [Google Scholar]
  • 5.Hodge M.B., Olivo H.F. Stereoselective aldol additions of titanium enolates of N-acetyl-4-isopropyl-thiazolidinethione. Tetrahedron. 2004;60:9397–9403. doi: 10.1016/j.tet.2004.08.008. [DOI] [Google Scholar]
  • 6.Ying Y., Liu Y., Byeon S.R., Kim H., Luesch H., Hong J. Synthesis and Activity of Largazole Analogues with Linker and Macrocycle Modification. Org. Lett. 2008;10:4021–4024. doi: 10.1021/ol801532s. [DOI] [PubMed] [Google Scholar]
  • 7.Seiser T., Kamena F., Cramer N. Synthesis and Biological Activity of Largazole and Derivatives. Angew. Chem. Int. Ed. 2008;47:6483–6485. doi: 10.1002/anie.200802043. [DOI] [PubMed] [Google Scholar]
  • 8.Ren Q., Dai L., Zhang H., Tan W., Xu Z., Ye T. Total Synthesis of Largazole. Synlett. 2008;15:2379–2383. [Google Scholar]
  • 9.Pattenden G., Thom S.M., Jone M.F. Enantioselective Synthesis of 2-alkyl substituted cysteines. Tetrahedron. 1993;49:2131–2138. doi: 10.1016/S0040-4020(01)86313-4. [DOI] [Google Scholar]
  • 10.Ghosh A.K., Kulkarni S. Enantioselective Total Synthesis of (+)-Largazole, a Potent Inhibitor of Histone Deacetylase. Org. Lett. 2008;10:3907–3909. doi: 10.1021/ol8014623. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 11.You S.L., Razavi H., Kelly J.W. A Biomimetic Synthesis of Thiazolines Using Hexaphenyloxodiphosphonium Trifluoromethanesulfonate. Angew. Chem. Int. Ed. 2003;42:83–85. doi: 10.1002/anie.200390059. [DOI] [PubMed] [Google Scholar]
  • 12.Numajiri Y., Takahashi T., Takagi M., Shin-ya K., Doi T. Total Synthesis of Largazole and Its Biological Evaluation. Synlett. 2008;16:2483–2486. [Google Scholar]
  • 13.Sakakura A., Kondo R., Ishihara K. Molybdenum Oxides as Highly Effective Dehydrative Cyclization Catalysts for the Synthesis of Oxazolines and Thiazolines. Org. Lett. 2005;7:1971–1974. doi: 10.1021/ol050543j. [DOI] [PubMed] [Google Scholar]
  • 14.Osorio-Lozada A., Olivo H.F. Indene-Based Thiazolidinethione Chiral Auxiliary for Propionate and Acetate Aldol Additions. Org. Lett. 2008;10:617–620. doi: 10.1021/ol702980p. [DOI] [PubMed] [Google Scholar]
  • 15.Nasveschuk C.G., Ungermannova D., Liu X., Phillips A.J. A Concise Total Synthesis of Largazole, Solution Structure, and Some Preliminary Structure Activity Relationships. Org. Lett. 2008;10:3595–3598. doi: 10.1021/ol8013478. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 16.Hantzsch A. Ueber den Eintritt der Halogene in das Molekül des Acetessigäthers. Chem. Ber. 1890;23:2339–2342. doi: 10.1002/cber.189002302101. [DOI] [Google Scholar]
  • 17.Wang B., Forsyth C.J. Total Synthesis of Largazole - Devolution of a Novel Synthetic Strategy. Synthesis. 2009;17:2873–2880. [Google Scholar]
  • 18.Bowers A., West N., Taunton J., Schreiber S.L., Bradner J.E., Williams R.M. Total Synthesis and Biological Mode of Action of Largazole: A Potent Class I Histone Deacetylase Inhibitor. J. Am. Chem. Soc. 2008;130:11219–11222. doi: 10.1021/ja8033763. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 19.Bowers A.A., West N., Newkirk T.L., Youngman A.E., Schreiber S.L., Wiest O., Bradner J.E., Williams R.M. Synthesis and Histone Deacetylase Inhibitory Activity of Largazole Analogs: Alteration of the Zinc-Binding Domain and Macrocyclic Scaffold. Org. Lett. 2009;11:1301–1304. doi: 10.1021/ol900078k. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 20.Bowers A.A., Greshock T.J., West N., Estiu G., Schreiber S.L., Wiest O., Willims R.M., Bradner J.E. Synthesis and Conformation−Activity Relationships of the Peptide Isosteres of FK228 and Largazole. J. Am. Chem. Soc. 2008;131:2900–2905. doi: 10.1021/ja807772w. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 21.Zeng X., Yin B., Hu Z., Liao C., Liu J., Li S., Li Z., Niclaus M.C., Zhou G., Jiang S. Total Synthesis and Biological Evaluation of Largazole and Derivatives with Promising Selectivity for Cancers Cells. Org. Lett. 2010;12:1368–1371. doi: 10.1021/ol100308a. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Molecules are provided here courtesy of Multidisciplinary Digital Publishing Institute (MDPI)

RESOURCES