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. Author manuscript; available in PMC: 2019 May 21.
Published in final edited form as: Nature. 2018 Nov 21;563(7733):652–656. doi: 10.1038/s41586-018-0743-5

Figure 4. Interactions among D3-CTH, D53, and D14.

Figure 4.

(a) Time-dependent degradation of GST-D53 and GST-D53-D2 in Arabidopsis seedlings of Col-0 and max2–1 mutant extracts, and ± recombinant D14 and either D3 or D3ΔCTH. MG132: proteasome inhibitor. (b) Size-exclusion chromatography analysis of the interaction between D53-D2 and D14-GR24 ± ASK1-D3 with SDS–PAGE analysis of the elution fractions. (c) GST-pulldown assay using recombinant GST-D14 and non-tagged D53-D2 ± D3-CTH and/or GR24. (d) Kinetics of YLG hydrolysis by D14 in the presence of D3-CTH at increasing concentrations. All experiments repeated thrice.