Table 1.
Proteins identified in the WT PLRV-host interactome that are known to localize to the outer membrane of organelles.
| N. benthamiana AP c | S. tuberosum AP d | ||||
|---|---|---|---|---|---|
| Organelle a | Host Protein b | Fold Enrichment e | SAINT f | Fold Enrichment e | SAINT f |
| Plastid | TOC75-III | 200.6 | 1 | +/− | 1 |
| TOC34 | 35.3 | 1 | +/− | 0.92 | |
| TOC159 | 33.4 | 1 | nd | nd | |
| TOC132 | +/− | 0.8 | nd | nd | |
| OEP16 | +/− | 0.84 | +/− | 0.64 | |
| OEP37 | +/− | 0.82 | nd | nd | |
| CHUP1 | +/− | 0.85 | nd | nd | |
| HSP12 | +/− | 0.98 | nd | nd | |
| Mitochondria | VDAC1 | +/− | 0.98 | 1.9 | 0.61 |
| DRP1E | 10.2 | 1 | 7.7 | 1 | |
| Nucleus | NOP56 | 55.7 | 0.96 | +/− | 0.64 |
| NOP5-2 | 37.8 | 1 | nd | nd | |
| AT-IMP | 53.9 | 0.93 | +/− | 0.23 | |
| KPNB1 | +/− | 0.94 | +/− | 0.44 | |
a Confirmed membrane localization of host proteins identified in complex with PLRV. b Protein symbol curated from NCBI or Uniprot for A. thaliana orthologue by sharing the most significant sequence identity to the N. benthamiana and S. tuberosum host protein identified in complex with WT PLRV. c Data from the N.benthamiana-PLRV interactome reported in Reference [27]. d Data from the S. tuberosum-PLRV interactome reported in Reference [31]. e Fold enrichment calculation of host protein levels in WT PLRV APs compared to negative controls based on spectral counting. +/− indicates host proteins detected in WT PLRV APs but not negative controls. f Significance Analysis of INTeractome (SAINT) probability score indicating interaction confidence. nd = not detected.