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. 2018 Oct 31;9(11):530. doi: 10.3390/genes9110530

Correction: Ruiz-Rodríguez et al. Gut Microbiota of Great Spotted Cuckoo Nestlings Is a Mixture of Those of Their Foster Magpie Siblings and of Cuckoo Adults. Genes 2018, 9, 381

Magdalena Ruiz-Rodríguez 1, Manuel Martín-Vivaldi 2,3, Manuel Martínez-Bueno 3,4, Juan José Soler 3,5,*
PMCID: PMC6265951  PMID: 30384509

The authors wish to make the following changes in their paper [1]. The hypervariable DNA region we sequenced was the V6-V8 rather than the V4 as we erroneously wrote in the printed version. For the corrected version, we have added information on the universal primer used, as well as the performed modifications to include the Illumina Nextera adapters and barcodes. Moreover, we have also modified Table 1 where SE and t-values were erroneously preceded by a negative sign and are now shown between parentheses.

Table 1.

Comparisons of gut microbiome of magpie and great spotted cuckoo nestlings and of great spotted cuckoo adults in terms of bacterial richness, α-diversity, composition (weighed and unweighed unifrac β-diversity) and abundance and prevalence of bacterial families.

VARIABLE CONSIDERED TYPE OF SAMPLE COMPARISONS
(A) (B) (C) ANOVA POST-HOC (Tukey tests)
Adult cuckoos Nestling cuckoos Nestling magpies
(N = 6) (N = 12) (N = 7) A vs. B A vs. C B vs. C
Mean Mean Mean F2,22 p p p p
(SE) (SE) (SE) (SE) (SE)
Bacterial Richness 73.8 123 52.3 14.42 0.0001 0.008 0.405 0.0003
(12.1) (8.5) (11.2) (0.19) (0.000008)
α-diversity (Shannon index) 3.92 4.45 2.66 6.50 0.006 0.585 0.106 0.005
(0.43) (0.31) (0.4) (0.03) (0.00001)
PERMANOVA POST-HOC (Pair-wise t-tests)
Microbiome composition Pseudo-F (SE) p (SE) p (t-value) p (t-value) p (t-value)
Weighed unifrac β-diversity 4.35 0.00022 0.559 0.0002 0.0001 (2.63)
(0.01) (0.00004) (0.85) (2.87)
Unweighed unifrac β-diversity 5.37 0.0001 0.0002 0.0007 0.0001
(0.07) (0.0001) (1.72) (2.47) (2.61)
Abundance of Families (Bray−Curtis distance matrices) 5.33 0.00013 0.58 0.0008 0.002 (2.94)
(0.03) (0.00002) (0.9) (3.00)
Prevalence of Families (Jaccard distance matrices) 6.43 0.0001 0.0034 0.0006 0.0001 (2.86)
(0.12) (0.00001) (1.62) (2.55)

Bold fonts highlight statistically significant results (p < 0.05).

The following information is about the primer used, in Section 2.3. and now reads:

“B969F (5′-AATGATACGGCGACCACCGAGATCTACAC-NNNNNNNN-TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGACGCGHNRAACCTTACC-3′) and BA1406R (5′-CAAGCAGAAGACGGCATACGAGAT-NNNNNNNN-GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGACGGGCRGTGWGTRCAA-3′), where the Nextera adaptors (L and R arm) are in normal font (to either side of the barcodes), the barcodes are represented by NNNNNNNN, and the specific primer regions are bold + underlined.”

The following table is the correct one:

The changes do not affect the scientific results. The manuscript will be updated and the original will remain online on the article webpage, with a reference to this Correction.

Reference

  • 1.Ruiz-Rodríguez M., Martín-Vivaldi M., Martínez-Bueno M., Soler J.J. Gut Microbiota of Great Spotted Cuckoo Nestlings is a Mixture of Those of Their Foster Magpie Siblings and of Cuckoo Adults. Genes. 2018;9:381. doi: 10.3390/genes9080381. [DOI] [PMC free article] [PubMed] [Google Scholar]

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