Table 1.
Primers and conditions used in the PCRs.
| Gene | Primer | Conditions | ||
|---|---|---|---|---|
| 18S | Fw: | 5′-ATG TGA AGT CAC TGT GCC AG-3′ | 95 °C 60 °C 72 °C |
10 s 10 s 12 s |
| Rv: | 5′-GTG TAA TCC GTC TCC ACA GA-3′ | |||
| IL-1ra | Fw: | 5′-GAA GAT GTG CCT GTC CTG TGT-3′ | 95 °C 60 °C 72 °C |
10 s 10 s 15 s |
| Rv: | 5′-CGC TCA GGT CAG TGA TGT TAA-3′ | |||
| IL10 | Fw: | 5′-AGA ACC TGA AGA CCC TCA GGC-3′ | 95 °C 60 °C 72 °C |
10 s 10 s 15 s |
| Rv: | 5′-CCA CGG CCT TGC TCT TGT T-3′ | |||
| IL1β | Fw: | 5′-GGA CAG GAT ATG GAG CAA CA-3′ | 95 °C 58 °C 72 °C |
10 s 10 s 15 s |
| Rv: | 5′-GGC AGA CTC AAA TTC CAG CT-3′ | |||
| NFκB | Fw: | 5′-AAA CAC TGT GAG GAT GGG ATC TG-3′ | 95 °C 60 °C 72 °C |
10 s 10 s 15 s |
| Rv: | 5′-CGA AGC CGA CCA CCA TGT-3′ | |||
| TLR4 | Fw: | 5′-GGT CAC CTT TTC TTG ATT CCA-3′ | 95 °C 60 °C 72 °C |
10 s 10 s 15 s |
| Rv: | 5′-TCA GAG GTC CAT CAA ACA TCA C-3′ | |||
| TNFα | Fw: | 5′-CCC AGG CAG TCA GAT CAT CTT CTC GGA A-3′ | 94 °C 63 °C 72 °C |
10 s 10 s 15 s |
| Rv: | 5′-CTG GTT ATC TCT CAG CTC CAC GCC ATT-3′ | |||
| IL6 | Fw: | 5′-ACC TGA ACC TTC CAA AGA TGG C-3′ | 95 °C 63 °C 72 °C |
10 s 10 s 15 s |
| Rv: | 5′-TCA CCA GGC AAG TCT CCT CAT TG-3′ | |||
The relative quantification was performed by standard calculations considering 2(−ΔΔCt). mRNA levels of sedentary males were arbitrarily referred to as 1. The expression of the target gene was normalized with respect to ribosomal 18S.