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. 2012 May 8;17(5):5404–5411. doi: 10.3390/molecules17055404

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© 2012 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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Effects of laminarin on NO production in RAW 264.7 mouse macrophages. After 24 h incubation with laminarin, NO productioninRAW 264.7 was measured by a Griess reaction assay. The normal group (Normal) was treated with media only. Polymyxin B (0.5 µg/mL), an inhibitor of lipopolysaccharide (LPS; 1 µg/mL), did not show the significant effect on laminarin-induced NO production (A and B), whereas laminarin-induced NO production was significantly inhibited by intracellular calcium chelator, BAPTA-AM (BAPTA; 10 µM) (C). Values are the mean + SEM of three independent experiments. *** p < 0.001 vs. Normal.