Table 3.
The concentration variation of ligustilide (19 and/or 20) and butylphthalide (39) in CX analyzed by HPLC.
| No. | Analytes | Extraction solvent | Extraction method | Analytical method | Stationary phase | Mobile phase | Flow rate (mL/min) | λmax(nm) | Content(mg/g) | Ref. |
|---|---|---|---|---|---|---|---|---|---|---|
| 1 | ligustilide | - | - | HPLC | Nova-Pak C18(3.9 mm ° 150 mm) | Methanol and water with 10% isopropanol (53:47) | 0.8 | 280 | 15.7 | [116] |
| 2 | ligustilide | Methanol | Sonication | HPLC | Luna 5 μm silica(150 mm ° 4.6 mm) | n-hexane:ethyl acetate:chloroform (92:3:5) | 0.8 | 320 | 15.27 ± 1.86 | [117] |
| 3 | ligustilide | Acetonitrile | Shaking up | RP-HPLC | Hypersil ODS2(4.6 mm ° 200 mm, 5 μm) | Methanol:acetonitrile:water (33:21:46) | 0.8 | 275 | 347.9(in volatile oil) | [118] |
| 4 | ligustilide | Ethanol | Reflux | HPLC | C18(4.0 mm ° 200 mm, 5 μm) | Acetonitrile:water (both contain 0.1% acetic acid) | 0.76 | 280 | 8.2 | [119] |
| 5 | ligustilide | 70% ethanol | Reflux | HPLC | ODS C18(4.6 mm ° 200 mm, 5 μm) | Methanol:water:acetic acid (75.0:24.8:0.2) | 1.0 | 326 | 5.672~5.821 | [120] |
| 6 | ligustilide | Ethanol | Sonication | HPLC | Alltima C18(4.6 mm × 150 mm, 5 μm) | Acetonitrile:water(60:40) | 1.0 | 350 | 7.40 | [121] |
| 7 | butylphthalide | Acetonitrile | Shaking up | RP-HPLC | Kromasil C18(250 mm × 4.6 mm, 5 μm) | sodium acetate (0.05 mol/L):acetonitrile(45:55) | 1.0 | 228 | 131.2~138.3(in volatile oil) | [122] |
| 8 | butylphthalide | Ethyl ether | Sonication | RP-HPLC | Kromasil C18(250 mm × 4.6 mm, 5 μm) | Acetonitrile:acetic acid (pH 4.0, 45:55) | 1.0 | 228 | 7.86~8.01 | [123] |