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Recycling experiments with Rh(III)-TsDPEN. Upper: time courses of the single experiments (1:
; 2:
; 3:
; 4:
; 5:
; 6:
; 7:
; 8:
; 9:
; 10:
); lower: initial rates. Conditions: 50 mg Rh(III)-TsDPEN (0.35 μmol), c(NAD+)0 = 0.25 mM, 50 mM phosphate buffer pH 7, T = 30 °C, c(NaHCO2) = 150 mM, 1,000 rpm; 100% corresponds to an NADH-generation rate of 0.21 mM·h−1.
