Figure 5.

miR-150 regulation by MYC in chronic myeloid leukemia (CML) primary cells. MYC occupancy at putative regulatory loci of the MIR150 gene in separated (A) CD34⁺ and (B) CD34⁻ cell subpopulations isolated from healthy donors [control (Ctrl) n=3] and CML-chronic phase (CP) patients at the time of diagnosis (CML; n=3), respectively. Separated cells from 2 healthy donors were pooled due to very low yields. The columns represent the fold change (FC) in the % of DNA input obtained in the leukemic or healthy cells compared with the non-specific IgG precipitation and equalized to 1 (dashed line). Unpaired two-tailed Student t-test was used to determine P-values. ^*P<0.05, ^**P<0.01. Error bars represent standard deviations.