Figure 3.

Immobilization reaction specificity of peptide N₃-Cy5-P1 versus non-specific binding (NSB) of peptide Cy5-P1 to ADIBO-functionalized glass slides. 1 μL of peptides of various concentrations were spotted. Fluorescence signal was recorded at λ = 635 nm after successive slide washing (left). A corresponding microarray image from one representative experiment is shown (right). Experiments were performed three times and data are mean values ± SD of triplicate measurements.