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. 2013 Aug 16;18(8):9833–9849. doi: 10.3390/molecules18089833

Figure 4.

Figure 4

Reaction kinetics of azido-functionalized peptides to ADIBO-modified glass slides. 1 μL of peptides N3-Cy5-myc, N3-Cy5-P1 and N3-Cy5-P2 diluted in H2O or H2O:DMSO at a 1:1 ratio were deposited and allowed react for the indicated time. Fluorescence signals were recorded at λ = 635 nm after slides washing (top). Determination of peptide saturation concentrations (bottom): 1 μL of peptides N3-Cy5-myc, N3-Cy5-P1 and N3-Cy5-P2 diluted in H2O at indicated concentrations were spotted and incubated for 30 min to allow immobilization, washed extensively for 3x three min with PBS 1X containing 0.05% of Tween-20, rinsed with H2O and dried under N2-flux. Kinetic and saturation experiments were performed twice and data are mean values ± SD of triplicate measurements.