Table 1.
Chaμracteristics of the 65 cDNA-SSR markers for common vetch (Vicia sativa subsp. sativa).
| Marker | Primer sequence (5'-3') | Motif | GenBank Acc. No. | Ta (°C) | BLAST top hit Acc. No. | Description | E-value |
|---|---|---|---|---|---|---|---|
| GBSSR-VSspS-020 | F: CATTTGGCTGATCCTGTCA R: GGCTTCATCATGAGACAAGAA |
(TAT)5 | KF008486 | 55 | L19651.1 | Pisum sativum chloroplast photosystem I 24 kDa light harvesting protein (lhca3) mRNA, complete cds | 7e-92 |
| GBSSR-VSspS-023 | F: CCTGCATTCACAACCATTT R: CGCCATCGATGTTTTGTT |
(CAT)5 | KF008487 | 55 | None | None | None |
| GBSSR-VSspS-024 | F: ACGGTGTTAACGGTCACG R: TCTCCAAACCGACACCAG |
(AGA)5 | KF008488 | 55 | AF262939.1 | Pisum sativum chloroplast protein import component Toc159mRNA. complete cds | 1e-160 |
| GBSSR-VSspS-028 | F: TGAGCCGTTGACACAACA R: GGCGATCCTCCTACTTGAA |
(TGC)5 | KF008489 | 55 | None | None | None |
| GBSSR-VSspS-037 | F: GAAACAAGCTGAAGGCCC R: TCAGGAAATGACCAAACCA |
(GAA)5 | KF008490 | 55 | X76774.1 | P.sativum mRNA for HMG1 protein | 2e-123 |
| GBSSR-VSspS-038 | F: CTCCCCAACTTGTTCCCT R: GGGAACTTGTCGATGTGG |
(TTC)5 | KF008491 | 55 | BT143793 | Medicago truncatula clone JCVI-FLMt-3P13 unknown mRNA | 3e-41 |
| GBSSR-VSspS-042 | F: GGTTCGAGAGCTTTGCTG R: CTGTGCCACTTGACCTCC |
(CGT)5 | KF008492 | 55 | JF965421.1 | Medicago sativa ethylene response factor 11 (ERF11) mRNA, complete cds | 2e-37 |
| GBSSR-VSspS-057 | F: GAGGTTTCCGGTGAGGAG R: GTTCCAGCAGGTGAAGCA |
(GGT)7 | KF008493 | 55 | XM_003548374.1 | PREDICTED: Glycine max DEAD-box ATP-dependent RNA helicase 31-like (LOC100799999), mRNA | 7e-28 |
| GBSSR-VSspS-066 | F: AGGAGAGGCAAGGACCAG R: CACGGCTATTTTCTTCTTTTTC |
(GA)10 | KF008494 | 55 | AB078603.1 | Pisum sativum SN4TDR mRNA for 110kDa 4SNc-Tudor domin protein, complet cds | 1e-93 |
| GBSSR-VSspS-067 | F: CAAACTTGTCACCACATATACAA R: GGTGGTCACTAGTGGAGGTG |
(CCA)5 | KF008495 | 55 | KC218790.1 | Vicia faba clone NAC-VF- 218 microsatellite sequence | 3e-39 |
| GBSSR-VSspS-071 | F: GTATTCCTCTGGTGGTGGG R: CACCACCAAGACCTCCAA |
(TGG)5 | KF008496 | 55 | XM 003617084.1 | Medicago truncatula Heterogeneous nuclear ribonucleoprotein DO (MTR 5g088220) mRNA, complete cds | 9e-108 |
| GBSSR-VSspS-073 | F: CCTCCCAATCCTCCATTC R: CCCTAGTCCTCCAATTTCG |
(GTT)5 | KF008497 | 55 | XM_004506417.1 | PREDICTED: Cicer arietinum scarecrow-like protein 6-like (LOC101497219), mRNA | 2e-58 |
| GBSSR-VSspS-075 | F: TTCAGCAAGCCCATCATT R: CGTCCGTCCAATCAACAA |
(TTA)6 | KF008498 | 55 | JF768700.1 | Lens culinaris microsatellite LcSSR535 sequence | 1e-71 |
| GBSSR-VSspS-076 | F: CCTGGTCCCAGAAATGGT R: AAGCCAGAGGGCATTGAT |
(CTG)6 | KF008499 | 55 | XM_003602555.1 | Medicago truncatula NAC domin protein (MTR_3g096140) mRNA complete cds | 1e-136 |
| GBSSR-VSspS-079 | F: AAAGCAAATTGTTAAAGAAAGGG R: GAGGATGCTGCACATATGTAGTT |
(AAT)5 | KF008500 | 55 | None | None | None |
| GBSSR-VSspS-080 | F: AATGCATGGATCGAGGTG R: GAATCCATCGGCAACGTA |
(TGG)5 | KF008501 | 55 | XM_004510745.1 | PREDICTED: Cicer arietinum uncharacterized LOC 101512367 (LOC 101512367), transcript variant X3, mRNA | 6e-110 |
| GBSSR-VSspS-088 | F: CGAAGAGGTAAATGACGCC R: AGTGACCTATATTTAGCATCGTT |
(TGG)5 | KF008502 | 55 | None | None | None |
| GBSSR-VSspS-090 | F: AGACGCACCACAACAGAAA R: GGGCTAGACATGGCACAA |
(AGC)6 | KF008503 | 55 | BT149661.1 | Medicago truncatula clone JCVI-FLMt-1507 unknown mRNA | 7e-62 |
| GBSSR-VSspS-091 | F: CCAAACCAGCAAGAGCAG R: GAGCAGCGTTGTCTCGTC |
(CTT)5 | KF008504 | 55 | XM_003594377.1 | Medicago trancatula Endoglucanase (MTR_2g028480) mRNA complete cds | e-122 |
| GBSSR-VSspS-099 | F: ATCCATGCCTCTTTTGCC R: AGCCTCATTTCAGCAGCA |
(TCT)5 | KF008505 | 55 | BT137674.1 | Medicago truncatula clone JCVIMt-1708 unknown mRNA | 3e-77 |
| GBSSR-VSspS-102 | F: TTCAACGGAGATGGATCG R: CGTCTTCTTTCAGAGGCG |
(GTT)5 | KF008506 | 55 | X59773.1 | Pisum sativum mRNA for P protein. a part of glycine cleavage complex | 0.0 |
| GBSSR-VSspS-107 | F: TGGTTTCTTTCTAAAGGGGTG R: CGGCTCGATGGACAGTAG |
(GTT)5 | KF008507 | 55 | BT146949.1 | Medicago truncatula clone JCVI-FLMt-19I1 unknown | e-127 |
| GBSSR-VSspS-115 | F: CATAAACAAGGGCAAGAAAA R: GAGGAAAACATTGGTGGGA |
(TGC)6 | KF008508 | 55 | XM_004501895.1 | PREDICTED: Cicer arietinum nucleobase- ascorbate transporter 6-like (LOC101504609), transcript X2, mRNA | 6e-59 |
| GBSSR-VSspS-117 | F: CGGTGCACTAAGTGGGAA R: TTAATGATGGTGGCGAGG |
(AGG)5 | KF008509 | 55 | BT135350.1 | Medicago truncatula clone JCVI-FLMt-9H4 unknown mRNA | 2e-114 |
| GBSSR-VSspS-118 | F: GCATTTCCCTTGGTCTCC R: CAGAAAGAGCAACCGTGC |
(TGG)5 | KF008510 | 55 | XM_004514512.1 | PREDICTED: Cicer arietinum N-alpha-acetyltransferase 10-like (LOC101504041), mRNA | 3e-68 |
| GBSSR-VSspS-119 | F: CACCACCAAGACCTCCAA R: CCATCATCATCACCAGCC |
(ACC)5 | KF008511 | 55 | XM_003617084.1 | Medicago truncatula Heterogeneous nuclear ribonucleoprotein D0 (MTR_5g088220), complete cds | 9e-98 |
| GBSSR-VSspS-125 | F: GGCCGGTATTCGTCAACT R: CCCCGTATTTTCTCGGTC |
(TGG)5 | KF008512 | 55 | XM_004510745.1 | PREDICTED: Cicer arietinum uncharacterized LOC 101512367 (LOC101512367), transcript variant X3, mRNA | 2e-134 |
| GBSSR-VSspS-126 | F: TGGCGCTTATCGCTATGT R: TCCACTCATTCCACTCGT |
(TG)7 | KF008513 | 55 | XM_003623363.1 | Medicago truncatula Patellin-6 (TR_7G070480) mRNA, complete cds | 5e-81 |
| GBSSR-VSspS-129 | F: AGGAGAGGCAAGGACCAG R: CTTTTTCTCTAACTCATTCATGTC |
(GA)10 | KF008514 | 55 | AB0786031 | Pisum sativum SN4TDR mRNA for 110kDa 4SNc-Tudor domain protein, complete cds | 8e-117 |
| GBSSR-VSspS-135 | F: TGGTGGAGATTTGTTGGG R: CTTCATCTTCCCACACCG |
(TGG)5 | KF008515 | 55 | BT136030.1 | Medicago truncatula clone JCVI-FLMt-16L14unknown mRNA | 4e-40 |
| GBSSR-VSspS-138 | F: CGGAGTTCACATAAAACATACTAC R: TGGGAGTGTTGAGATGGG |
(TTA)7 | KF008516 | 55 | AB176563.1 | Vicia faba MET mRNA for type 2 metallothionein, complete cds | 6e-98 |
| GBSSR-VSspS-140 | F: TTGCTTTGATGTTTGGAGC R: CCCTAAATTCCCAACCCA |
(GGT)7 | KF008517 | 55 | XM_003613856.1 | Medicago truncatula Cysteine-rich receptor-like protein kinase (MTR_5g042440) mRNA, complete cds | 3e-86 |
| GBSSR-VSspS-156 | F: GGCCAATTTAGCGAGCTT R: CACTATCATCAACCTCTAACGGA |
(GTG)5 | KF008519 | 55 | BT134176.1 | Medicago truncatula clone JCVI-FLMt-15D24 unknown mRNA | 2e-118 |
| GBSSR-VSspS-158 | F: TGAGCTTATTGCCAGTGGA R: CCATGTCATCATCGGATTC |
(TGG)5 | KF008520 | 55 | KC218603.1 | Vicia faba clone NAC-VF-31 microsatellite sequence | 7e-124 |
| GBSSR-VSspS-162 | F: GAGACAGTGGAAGTATCGGC R: CACAGCAAATGCATCGGT |
(AAG)6 | KF008521 | 55 | BT146412.1 | Medicago truncatula clone JCVI-FLMt-21014 unknown mRNA | 1e-75 |
| GBSSR-VSspS-166 | F: GTGGCCATGATCCATTTG R: TTCCTCGAGAGGGAAAGC |
(TGG)5 | KF008522 | 55 | XM_003605932.1 | Medicago truncatula DnaJ (MTR_4g50420) mRNA complete cds | 0.0 |
| GBSSR-VSspS-172 | F: GCTTTGGAAGAGCCCAAT R: TCCAGGATTGTAACCCCC |
(TGG)5 | KF008523 | 55 | XM_003617084.1 | Medicago truncatula Heterogeneous nuclear ribonucleoprotein D0 (MTR_5g088220) mRNA, complete cds | 5e-90 |
| GBSSR-VSspS-173 | F: GGGCACGGTGGTCACTA R: TGACTACCACCACCTCCG |
(TGG)5 | KF008524 | 55 | AJ831469.1 | Pisum sativum mRNA for putative glycine rich protein precursor (grp1 gene) | 2e-13 |
| GBSSR-VSspS-179 | F: AGCTATGCGAGAGGCTCC R: CTGTGGGAAGGCACATCT |
(TGA)6 | KF008525 | 55 | None | None | None |
| GBSSR-VSspS-181 | F: CACTGTGACTCAGTTTCGTTG R: CGATTTTGAACCCTAACCG |
(TTC)5 | KF008526 | 55 | None | None | None |
| GBSSR-VSspS-182 | F: GCGTTGTGGCGTATTTCT R: TGGAGGAAAGGAAACTACTCA |
(GCA)6 | KF008527 | 55 | AB676029.1 | Lathyrus japonicas DNA, 61 locus, haplotype: D | 4e-62 |
| GBSSR-VSspS-185 | F: CTCCTCAATTTTCCCCCA R: TTTGGTGCGATTGTTTCC |
(CAT)5 | KF008528 | 55 | Ap009676.1 | Lotus japonicas genomic DNA, clone: LjT30I08, TM0492 | 4e-26 |
| GBSSR-VSspS-187 | F: CCAGGTTGCTTTCCTTACTTT R: TTAGCCCTCAAAGCCTCC |
(ATC)5 | KF008529 | 55 | X54359.1 | P.sativum mRNA of cDNA clone 26g | 2e-154 |
| GBSSR-VSspS-192 | F: AGGGTCTTCCTTCCCACA R: TATGGTGACACGTTCGCA |
(ATC)5 | KF008530 | 55 | XM_004505980.1 | PREDICTED: Cicer arietinum uncharacterized LOC101500025 (LOC101500025), transcript variant X2, mRNA | 1e-100 |
| GBSSR-VSspS-203 | F: TCCATCTGGTTGGTGGTG R: GAAAGCCAATTTTTCAGCAA |
(GTT)7 | KF008531 | 55 | BT147294.1 | Medicago truncatula clone JCVI-FLMt-15A20 unknown mRNA | 2e-50 |
| GBSSR-VSspS-217 | F: CCATCGCCACCACCA R: TCCCGGAACAAAAATCAA |
(AAC)7 | KF008532 | 55 | EF447278.1 | Pisum sativum cultivar Finale SYM8 (SYM8) gene, partial cds | 5e-58 |
| GBSSR-VSspS-245 | F: CAATAGGGGGACCCTTCA R: GCTGCAAGCTGCTACCAT |
(GGA)5 | KF008533 | 55 | HQ439603.1 | Phalaenopsis hybrid cultivar candidate developmental transcription factor TCP1 mRNA, partial cds | 5e-04 |
| GBSSR-VSspS-247 | F: GGTTCAATACGATCCATAGAATA R: TGATCGCCAATTCTGGAC |
(CAC)6 | KF008534 | 55 | KC294548.1 | Aeschynomene ciliate voucher IRRI 13078 cyclophilin 1 (CYP1) gene, complete cds | 3e-46 |
| GBSSR-VSspS-249 | F: AAAACATGGTTGAGTGTTTTTG R: TAACCCTCTCGGTTTCGG |
(ATA)5 | KF008535 | 55 | KC218749.1 | Vicia faba clone NAC-VF-177 microsatellite sequence | 2e-55 |
| GBSSR-VSspS-251 | F: TGGTGGACGTCACTATGGA R: CATGGTGCTTCCGACAAT |
(TGG)5 | KF008536 * | 55 | KC218790.1 | Vicia faba clone NAC-VF-218 microsatellite sequence | 5e-30 |
| GBSSR-VSspS-252 | F: CATGGTGCTTCCGACAAT R: TCGAAATCAGGACTTACCACA |
(CCA)5 | KF008536 * | 55 | KC218790.1 | Vicia faba clone NAC-VF-218 microsatellite sequence | 5e-30 |
| GBSSR-VSspS-262 | F: ATTGGGCCCTCTTTTTGA R: GGGGGTAGAAAAGTTGCG |
(AT)7 | KF008537 | 55 | XM_004500781.1 | PREDICTED: Cicer arietinum dolichyl- diphoshooligosaccharide-protein glycosyltransferase subunit 1A-like (LOC101502563), mRNA | 1e-37 |
| GBSSR-VSspS-268 | F: AAATTTGTCTGACGAAAAACG R: TGCTTGAGAGTGCCATCA |
(TAC)5 | KF008538 | 55 | BT144509.1 | Medicago truncatula clone JCVI-FLMt-13M6 unknown mRNA | 2e-47 |
| GBSSR-VSspS-269 | F: TTCCATTTATCCTCCTATCCTCT R: CTTGAATGCGAAACGAGG |
(CGC)5 | KF008539 | 55 | XM_004507695.1 | PREDICTED: Cicer arietinum eukaryotic translation initiation factorisoform 4G-1-like (LOC101492356), mRNA | 1e-56 |
| GBSSR-VSspS-284 | F: TGGAAGGAAATGGCAGTG R: ATCCGTTTCGGATTGGTT |
(GCA)5 | KF008540 | 55 | JX539287.1 | Vigna radiata cultivar MCV-1 clone GGSSR_911 microsatellit sequence | 2e-120 |
| GBSSR-VSspS-291 | F: CCCAACCGAACCACTTATT R: TAATAGCTCCGGCCCAGT |
(CTA)5 | KF008541 | 55 | None | None | None |
| GBSSR-VSspS-301 | F: AACCAAACAACAATGGGTT R: TCAACCGGTGAAAGATGG |
(CAA)5 | KF008542 | 55 | JN849865.1 | Medicago falcata voucher PI494662A caffeic acid-O- methyltransferase (COMT) gene.exon 1 and partial cds | 2e-128 |
| GBSSR-VSspS-304 | F: CCGTTCTACGCAATTCTCC R: CGACCAAGAACACCAGGA |
(TTC)5 | KF008543 | 55 | XM_003593951.1 | Medicago truncatula hypothetical protein (MTR_2g020190) mRNA,complete cds | 1e-55 |
| GBSSR-VSspS-305 | F: CATGAAAGAGTTTTGCACCTT R: CCGACGACGAGATTGAGA |
(GCA)5 | KF008544 | 55 | None | None | None |
| GBSSR-VSspS-308 | F: TGAGAGCATAGACAGCAAACA R: TGGATTTGGTCGCATAGC |
(AAC)5 | KF008545 | 55 | None | None | None |
| GBSSR-VSspS-309 | F: TCTTCAAAAGAGTACAAAAGGGA R: GAATTGGACACCTTGGCA |
(AAT)5 | KF008546 | 55 | BT137399.1 | Medicago truncatula clone JCVI-FlMt-19L22 unknown mRNA | 5e-124 |
| GBSSR-VSspS-310 | F: GGGTGCCCTAGCATTTGT R: ATCTCCGGCGTCAGTTTC |
(CTC)6 | KF008547 | 55 | M69105.1 | Pisum sativum outer membrane protein (OM14) mRNA, complete cds | 9e-96 |
| GBSSR-VSspS-311 | F: TTGAGGCGGTGTTGGTAG R: ATGTCATGGCCAACTGCT |
(GGA)6 | KF008548 | 55 | None | None | None |
| GBSSR-VSspS-313 | F: GAACAATGCAGCCTGGAA R: GCTGCAATCGCATTCTCT |
(TTG)5 | KF008549 | 55 | XM_003613196.1 | Medicago truncatula U-box domin containing protein (MTR_5g034440) mRNA, complete cds | 2e-112 |
TA, annealing temperature. * same sequence two primers identified.