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. 2013 Nov 13;18(11):14042–14069. doi: 10.3390/molecules181114042

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© 2013 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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Imaging of macrophage burden using an off-resonance imaging sequence (IRON, inversion recovery with ON-resonant water suppression). (A) Before the injection of MION tissue is homogeneously suppressed using the IRON sequence. (B) 48 h after the injection of MION, intraplaque iron oxide particles generate areas of positive contrast on IRON images, which were in good agreement with areas of iron deposition on corresponding histological slices. The magnitude of positive signal enhancement correlated with the number of macrophages determined by histology. MION: Magnetic iron oxide nanoparticle (adapted from Korosoglou) [80].