Figure 3.

A representative result of sensing current responses by testing the glutamate sensor prepared by Method 2 (▬ black line) and the control sensor (▬ gray line) with dopamine (5 μM and 10 μM), ascorbic acid (250 μM and 500 μM), glutamate (20 μM, 40 μM, 240 μM), and hydrogen peroxide (20 μM), sequentially. The injection of dopamine is indicated by the solid black arrow, the injection of ascorbic acid is indicated by the dashed black arrow, the injection of glutamate is indicated by the solid gray arrow, and the injection of hydrogen peroxide is indicated by the dashed gray arrow. The control sensor was prepared by the same sensor fabrication process as that of the glutamate sensor except that the control sensor does not have the enzyme layer.