Figure 5.

Effect of fisetin on HO-1 expression in microglia cells. (A) Cells were treated with fisetin, LPS (10 ng/mL) plus IFN-γ (10 ng/mL), or fisetin with LPS/IFN-γ for 24 h. Cells were treated with different concentrations of fisetin (1, 3, or 5 μM) for 24 h (B) or 6 h (C). Whole cell lysates were subjected to western blot analysis for HO-1 protein expression (A,B). HO-1 mRNA expressions were analyzed by real time-PCR (C). Cells were treated with Copp IX for 60 min, then challenged with LPS plus IFN-γfor another 24 h. The expression of iNOS and HO-1 were determined by western blot analysis (D). NO production was analyzed by Griess reaction (E). The results are expressed as mean ± S.E.M. from 3 to 4 independent experiments. * p < 0.05 compared with the control group; ^# p < 0.05 compared with the LPS/IFN-γ treatment alone group.