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Effects of 3,6-dihydroxyflavone (3,6-DHF) on cyclooxygenase-2 (COX-2), phospho-p38, phospho-extracellular-signal-regulated kinase (ERK), and phospho-Jun-N terminal kinase (JNK) in human tumor necrosis factor alpha (hTNF-α)-stimulated HeLa cells. COX-2, phospho-p38, phospho-ERK, phospho-JNK, and β-actin (loading control) were determined by western blot analysis using specific antibodies. The relative mRNA expression was quantified using Image J (NIH, Bethesda, MD, USA).
