. 2014 Sep 12;19(9):14461–14483. doi: 10.3390/molecules190914461

Table 2.

Examples of the application of NCL to circumvent peptide size limitation. Cysteine residues used as ligation sites are in bold and larger font. γ = Gamma carboxyglutamic acid.

Toxin/Peptide	Application	Sequence	Ref.
Human interleukin 8 (IL-8)	Chemokine, chemoattractants for leukocytes	SAKELRCQCIKTYSKPFHPKFIKELRVIESGPHCANTEIIVKLSDGRELCLDPKEWVQRVVEKFLKRAENS	[5,90]
Human group II secretory phospholipase A₂ (sPLA₂)	Hydrolysis of the fatty acid side chain ester bond	NLVNFHRMIKLTTGKEAALSYGFYGCHCGVGGRGSPKDATDRCCVTHDCCYKRLEKRGCGTKFLSYKFSNSGSRITCAKQDSCRSQLCECDKAAATCFARNKTTYNKKYQYYSNKHCRGSTPRC	[22]
Microprotein S	Anticoagulant cofactor activity	NSLLγγTKQGNLγRγCIγγLCNKγγARγVFγNDPγTDYFYPKYLGCLRSFQTGLFTAARQSTNAYPDLRSCVNAIPDQCSPLPCNEDGYMSCKDGKASFTCTCKPGWQG EKCEFD	[89]
Barnase (Lys⁴⁹-Cys⁴⁹)	Microbial ribonuclease	AQVINTFDGVADYLQTYHKLPNDYITKSEAQALGWVASKGNLADVAPGCSIGGDIFSNREGKLPGKSGRTWREADINYTSGFRNSDRILYSSDWLIYKTTDHYQTFTKIR	[24]