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. 2014 Sep 12;19(9):14461–14483. doi: 10.3390/molecules190914461

Table 4.

Examples of the applications of NCL to synthesize glycopeptides and glycoproteins. Cysteine residues used as ligation sites are in bold and larger font. Glycosylation sites are shown with underlining residues.

Toxin/Peptide Application Sequence Ref.
Diptericin ε (Cys25,Glu29,Glu45) Antibacterial glycopeptide DEKPKLILPTPAPPNLPQLVGGGGCNRKEGFGVSVDAHQKVWTSENGRHSIGVTPGYSQHLGGPYGNSRPDYRIGAGYSYNF [109]
Lymphotactin (Lptn) Chemoattractant (for T- cell and natural killer cell) VGSEVSDKRTCVSLTTQRLPVSRIKTYTITEGSLRAVIFITKRGLKVCADPQATWVRDVVRSMDRKSNTRNNMIQTKPTGTQQSTNTAVTLTG [112]
RNase B Fragment Cleavage of N-linked carbohydrates MKSRNLTKDRCKPVNTFVHESLADVQAVCSQKNVACKNG [115]
CCL7 (MCP-3) Monocyte specific chemotactic protein-3 QPVGINTSTTCCYRFINKKIPKQRLESYRRTTSSHCPREAVIFKTKLDKEICADPTQKWVQDFMKHLDKKTQTPKL [116]