Figure 1.

Effect of hispidin and palmitate on C2C12 myotubes viability. (A) Cytotoxic effect of hispidin on C2C12 myotubes. Differentiated cells were treated with various concentrations of hispidin for 24 h. The cytotoxicity of palmitate was determined after exposure to various concentrations of palmitate for 16 h; (B) and different exposure times with 1 mM palmitate; (C). C2C12 myotubes viability was measured by MTT assay. The cells treated with only BSA conditioned medium were used as a control. Palmitate was administered by conjugating it to BSA (fatty acid free). Data represent the mean ± standard error of three independent experiments. * p < 0.05, ** p < 0.01, significantly different from the control group or only BSA-treated group. CON, control; BSA, bovine serum albumin.