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. 2015 Mar 27;20(4):5456–5467. doi: 10.3390/molecules20045456

Figure 2.

Figure 2

Protective effect of hispidin in palmitate-damaged C2C12 myotubes. (A) Effect of hispidin on palmitate-induced cell death in C2C12 myotubes. Cells were treated with 1 to 200 μM hispidin for 8 h, and then 1 mM palmitate was added for 16 h. Cell viability was determined by the MTT assay. Data represent the mean ± standard error of three independent experiments. * p < 0.05, as compared with only BSA group, # p < 0.05, as compared with PA group. (B) Effect of hispidin on palmitate-induced cell loss in C2C12 myotubes. Cells were treated with hispidin 50 μM for 8 h, and then 1 mM palmitate was added for 16 h. Cell loss was determined by crystal violet staining. (C) The number of myotubes per photograph was counted after crystal violet staining using the Image J densitometry software program. The cells treated with only BSA conditioned medium were used as a control. Palmitate was administered by conjugating it to BSA (fatty acid free). BSA, bovine serum albumin; PA, palmitate.