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Human skin primary irritation test.
| No. | Test Material | 48 h | 72 h | Reaction grade b | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ± | 1+ | 2+ | 3+ | 4+ | ± | 1+ | 2+ | 3+ | 4+ | 48 h | 72 h | Mean | ||
| 1 | Squalene | - a | - | - | - | - | - | - | - | - | - | 0 | 0 | 0 |
| 2 | Silibinin (0.1%) | - | - | - | - | - | - | - | - | - | - | 0 | 0 | 0 |
a No reaction; b Reaction grade = ∑ [{Grade × No. of Responders}/{4 (Maximum grade) × 30 (Total Subjects)}] × 100 × (1/2).
