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(A) Verification of pAzF incorporation into TsFDH by MALDI-TOF MS; mass spectra of one tryptic digest of TsFDH-V13pAzF (residues 4-18; ILCVLYDDP pAzFDGYPK) (top) and TsFDH-WT (residues 4-18; ILCVLYDDPVDGYPK) (bottom) and (B) in-gel fluorescence of TsFDH-WT and TsFDH-V13pAzF obtained by the SPAAC-mediated DBCO-dye labeling.
